de Groot H J
Leiden Institute of Chemistry, Gorlaeus Laboratories, POB 9502, 2300 RA, Leiden, Netherlands.
Curr Opin Struct Biol. 2000 Oct;10(5):593-600. doi: 10.1016/s0959-440x(00)00136-6.
One major remaining problem in structural biology is to elucidate the structure and mechanism of function of membrane proteins. On the basis of preliminary information from genome projects, it is now estimated that up to 50,000 different membrane proteins may exist in the human being and that virtually every life process proceeds, sooner or later, through a membrane protein. Solid-state NMR spectroscopy in high magnetic field is rapidly developing into a widely applicable tool to describe the structure and help understand the mechanism of function of a membrane protein. Recent work in applied solid-state NMR spectroscopy crosses the boundary between the biological and the physical sciences, and aims at increasing the predictive range of this biophysical method.
结构生物学中一个尚待解决的主要问题是阐明膜蛋白的结构和功能机制。根据基因组计划的初步信息，目前估计人类可能存在多达50,000种不同的膜蛋白，而且几乎每个生命过程迟早都要通过膜蛋白来进行。高磁场固态核磁共振光谱正迅速发展成为一种广泛适用的工具，用于描述膜蛋白的结构并帮助理解其功能机制。应用固态核磁共振光谱的最新研究跨越了生物科学和物理科学的界限，旨在扩大这种生物物理方法的预测范围。
