Shaffer A L, Rosenwald A, Hurt E M, Giltnane J M, Lam L T, Pickeral O K, Staudt L M
Metabolism Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 4N114, Bethesda, MD 20892, USA.
Immunity. 2001 Sep;15(3):375-85. doi: 10.1016/s1074-7613(01)00194-7.
A compendium of global gene expression measurements from DNA microarray analysis of immune cells identifies gene expression signatures defining various lineages, differentiation stages, and signaling pathways. Germinal center (GC) B cells represent a discrete stage of differentiation with a unique gene expression signature. This includes genes involved in proliferation, as evidenced by high expression of G2/M phase regulators and low expression of ribosomal and metabolic genes that are transcriptional targets of c-myc. GC B cells also lack expression of the NF-kappaB signature genes, which may favor apoptosis. Finally, the transcriptional repression signature of BCL-6 reveals how this factor can prevent terminal differentiation of B cells and cause B cell lymphomas.
一份来自免疫细胞DNA微阵列分析的全球基因表达测量纲要，确定了定义各种谱系、分化阶段和信号通路的基因表达特征。生发中心（GC）B细胞代表了一个具有独特基因表达特征的离散分化阶段。这包括参与增殖的基因，G2/M期调节因子的高表达以及核糖体和代谢基因的低表达证明了这一点，而核糖体和代谢基因是c-myc的转录靶点。GC B细胞也缺乏NF-κB特征基因的表达，这可能有利于细胞凋亡。最后，BCL-6的转录抑制特征揭示了该因子如何防止B细胞终末分化并导致B细胞淋巴瘤。
