Yusaf S P, Goodman J, Pinnock R D, Dixon A K, Lee K
Pfizer Global Research and Development, Cambridge University Forvie Site, CB2 2QB, Cambridge, UK.
Neurosci Lett. 2001 Sep 28;311(2):137-41. doi: 10.1016/s0304-3940(01)02038-9.
In the present study, we have used in situ hybridisation to examine the distribution of calcium channel subunits in rat dorsal root ganglion (DRG) neurons. Within DRG neurons, the calcium channel alpha subunit mRNAs alpha(1A), alpha(1B), alpha(1C), alpha(1D), alpha(1E), alpha(1I) and alpha(1S) were readily detected in small (<25 microm), medium (25-45 microm) and large (>45 microm) diameter neurons. alpha(1F) was present at very low levels in these neurons whilst alpha(1G) was virtually undetectable. The calcium channel auxiliary subunits alpha(2)delta(1) and alpha(2)delta(2) showed a complementary pattern of distribution to that of alpha(2)delta(3) in DRG neurons. alpha(2)delta(1) and alpha(2)delta(2) transcripts were expressed predominantly in small c-type sensory neurons and were present at lower levels in large Abeta-type sensory neurons. In contrast, alpha(2)delta(3) mRNA was present in high quantities in the large-diameter cells but was expressed at lower levels in small-diameter neurons of the DRG. The present study provides an insight into the molecular profile of calcium channel alpha(1) and alpha(2)delta subunits in the neurons responsible for transmitting sensory information.
在本研究中，我们使用原位杂交技术检测大鼠背根神经节（DRG）神经元中钙通道亚基的分布。在DRG神经元内，钙通道α亚基mRNAα(1A)、α(1B)、α(1C)、α(1D)、α(1E)、α(1I)和α(1S)在小直径（<25微米）、中等直径（25 - 45微米）和大直径（>45微米）的神经元中均易于检测到。α(1F)在这些神经元中的表达水平非常低，而α(1G)几乎无法检测到。钙通道辅助亚基α(2)δ(1)和α(2)δ(2)在DRG神经元中的分布模式与α(2)δ(3)互补。α(2)δ(1)和α(2)δ(2)转录本主要在小的c型感觉神经元中表达，在大的Aβ型感觉神经元中的表达水平较低。相反，α(2)δ(3)mRNA在大直径细胞中大量存在，但在DRG的小直径神经元中的表达水平较低。本研究为负责传递感觉信息的神经元中钙通道α(1)和α(2)δ亚基的分子概况提供了深入了解。
