硒缺乏时胃肠道谷胱甘肽过氧化物酶信使核糖核酸的稳定性取决于其3'非翻译区。

Stability of gastrointestinal glutathione peroxidase mRNA in selenium deficiency depends on its 3'UTR.

作者信息

Wingler K, Müller C, Brigelius-Flohé R

机构信息

German Institute of Human Nutrition and Institute of Nutritional Science, University of Potsdam, Potsdam-Rehbrücke, Germany.

出版信息

Biofactors. 2001;14(1-4):43-50. doi: 10.1002/biof.5520140107.

DOI:10.1002/biof.5520140107

PMID:11568439

Abstract

Selenoproteins decrease upon selenium-deprivation according to their hierarchical ranking. Whereas classical glutathione peroxidase (cGPx) responds to decreased selenium supply with a complete loss of protein and a marked reduction of mRNA levels, gastrointestinal glutathione peroxidase (GI-GPx) remains detectable and its mRNA is stable. The impact of the 3'UTR on cGPx and GI-GPx mRNA stability was studied in stably transfected HepG2 cells with combinations of mutually exchanged coding regions and 3'UTRs of human cGPx and GI-GPx. Stability of chimeric mRNAs was measured by competitive RT-PCR. We found that GI-GPx 3'UTR is sufficient to stabilize its own mRNA but not that of cGPx.

摘要

根据硒蛋白的等级排序，在缺硒时其表达量会降低。经典谷胱甘肽过氧化物酶（cGPx）会因硒供应减少而出现蛋白质完全缺失和mRNA水平显著降低的情况，而胃肠道谷胱甘肽过氧化物酶（GI-GPx）仍可检测到，且其mRNA稳定。利用人cGPx和GI-GPx相互交换的编码区和3'非翻译区（3'UTR）组合，在稳定转染的HepG2细胞中研究了3'UTR对cGPx和GI-GPx mRNA稳定性的影响。通过竞争性逆转录聚合酶链反应（RT-PCR）测定嵌合mRNA的稳定性。我们发现，GI-GPx的3'UTR足以稳定其自身的mRNA，但不能稳定cGPx的mRNA。