Wong-Riley M T, Bai X, Buchmann E, Whelan H T
Department of Cell Biology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Neuroreport. 2001 Oct 8;12(14):3033-7. doi: 10.1097/00001756-200110080-00011.
Light close to and in the near-infrared range has documented benefits for promoting wound healing in human and animals. However, mechanisms of its action on cells are poorly understood. We hypothesized that light treatment with a light-emitting diode array at 670 nm (LED) is therapeutic in stimulating cellular events involving increases in cytochrome oxidase activity. LED was administered to cultured primary neurons whose voltage-dependent sodium channels were blocked by tetrodotoxin. The down-regulation of cytochrome oxidase activity by TTX was reverted to control levels by LED. LED alone also up-regulated enzyme activity. Thus, the results are consistent with our hypothesis that LED has a stimulating effect on cytochrome oxidase in neurons, even when they have been functionally silenced by TTX.
接近可见光及近红外范围的光已被证明对促进人和动物的伤口愈合有益。然而，其对细胞的作用机制却鲜为人知。我们推测，用670纳米发光二极管阵列（LED）进行光疗对刺激细胞活动具有治疗作用，这种细胞活动包括细胞色素氧化酶活性的增加。将LED应用于培养的原代神经元，这些神经元的电压依赖性钠通道被河豚毒素阻断。河豚毒素对细胞色素氧化酶活性的下调作用被LED恢复到对照水平。单独使用LED也上调了酶活性。因此，这些结果与我们的假设一致，即即使神经元已被河豚毒素功能性沉默，LED对神经元中的细胞色素氧化酶仍具有刺激作用。
