Tacconi G, Pedini A V, Gargiulo A M, Coletti M, Piergili-Fioretti D
Dipartimento di Scienze Biopatologiche Veterinarie, Perugia, Italy.
Avian Dis. 2001 Jul-Sep;45(3):688-95.
The morphometric characteristics and the ultramicroscopic findings of Cryptosporidium spp. at various stages of their life cycle in the intestinal and bursal epithelial cells of naturally infected 30-day-old commercial turkeys are reported. Small, sporulated oocysts, observed in the small intestinal content after flotation, were identified as Cryptosporidium meleagridis on the basis of morphometric characteristics (round in shape and 4.5-5.0 microm in size) and the small intestinal localization. Light section examinations revealed the presence of the protozoon in multiple organs, but its prevalence was highest in the intestinal and bursal epithelial cells. Ultramicroscopic studies on ileum and bursal samples showed the presence of all the life cycle stages in the microvillar brush epithelial cells in both the organs examined. On the basis of the comparison of the morphology and the sizes of the microorganisms parasitizing the ileum and the bursa, hypotheses are considered on the possible species involved.
报告了自然感染的30日龄商品火鸡肠道和法氏囊上皮细胞中隐孢子虫属在其生命周期各个阶段的形态测量特征和超微结构发现。通过浮选在小肠内容物中观察到的小的、有孢子的卵囊，根据形态测量特征（圆形，大小为4.5-5.0微米）和小肠定位，被鉴定为火鸡隐孢子虫。光镜切片检查显示该原生动物存在于多个器官中，但其在肠道和法氏囊上皮细胞中的感染率最高。对回肠和法氏囊样本的超微结构研究表明，在所检查的两个器官的微绒毛刷状上皮细胞中存在所有生命周期阶段。基于对寄生在回肠和法氏囊中的微生物的形态和大小的比较，考虑了可能涉及的物种的假设。
