Bochenek M, Smorag Z, Pilch J
Department of Animal Reproduction, National Research Institute of Animal Production, Balice/Kraków, Poland.
Theriogenology. 2001 Sep 1;56(4):557-67. doi: 10.1016/s0093-691x(01)00588-x.
The goal of our study was to find the relationship between fertility of bulls qualified for AI and the percentage of spermatozoa with abnormal chromatin structure as an independent parameter. We used the frozen semen of 8 mature bulls from one AI center. Each bull was represented by 3 ejaculates collected with at least 2-week intervals. Bull fertility was calculated on the basis of non-return ratio and was expressed as a scale where 100 points represented the average fertility of all the AI center's bulls. Bulls with lower or higher fertility received a lower or higher score respectively. Fertility scores of bulls used in the study ranged from 83 to 104 . Semen was processed according to the SCSA (sperm chromatin structure assay) method and was analyzed by flow cytometry. "Artificial" alpha(t) (alpha(t)=red/green+red fluorescence) and red fluorescence histograms were used for calculation of COMPalpha(t), SDalpha(t), %Red, %PeakR and MeanR parameters. The percentage of spermatozoa with abnormal chromatin ranged from 1.2% to 23.8%. A large variation among ejaculates was found for bulls with lower fertility. Fertility correlated significantly with COMPalpha(t) (-0.50, P < 0.05), SDalpha(t) (-0.55, P < 0.01), %Red (-0.53, P < 0.01), %PeakR (-0.58, P < 0.01) and MeanR (-0.45, P < 0.05). The SCSA method has a practical application in analyzing spermatogenesis disorders in bulls. If regularly applied, it allows us to identify and eliminate ejaculates with a high level of sperm chromatin abnormalities.
我们研究的目的是找出符合人工授精条件的公牛的生育能力与作为独立参数的染色质结构异常的精子百分比之间的关系。我们使用了来自一个人工授精中心的8头成年公牛的冷冻精液。每头公牛由至少间隔2周采集的3次射精样本代表。公牛的生育能力根据不返情率计算，并以一个量表表示，其中100分代表该人工授精中心所有公牛的平均生育能力。生育能力较低或较高的公牛分别获得较低或较高的分数。本研究中使用的公牛的生育能力得分在83至104之间。精液按照精子染色质结构分析（SCSA）方法进行处理，并通过流式细胞术进行分析。使用“人工”α(t)（α(t)=红色/绿色+红色荧光）和红色荧光直方图来计算COMPα(t)、SDα(t)、%红色、%峰值R和平均R参数。染色质结构异常的精子百分比在1.2%至23.8%之间。生育能力较低的公牛的射精样本之间存在很大差异。生育能力与COMPα(t)（-0.50，P<0.05）、SDα(t)（-0.55，P<0.01）、%红色（-0.53，P<0.01）、%峰值R（-0.58，P<0.01）和平均R（-0.45，P<0.05）显著相关。SCSA方法在分析公牛精子发生障碍方面具有实际应用价值。如果定期应用，它使我们能够识别和排除精子染色质异常水平高的射精样本。
