印尼国家人工授精中心将牛精蛋白作为精液产量和质量蛋白质标志物的潜力。
The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia.
作者信息
Pardede Berlin Pandapotan, Maulana Tulus, Kaiin Ekayanti Mulyawati, Agil Muhammad, Karja Ni Wayan Kurniani, Sumantri Cece, Supriatna Iman
机构信息
Reproductive Biology Study Program, Faculty of Veterinary Medicine, IPB University, Dramaga, Bogor 16680, Indonesia.
Animal Reproduction Biotechnology Research Group, Research Center for Biotechnology, Indonesian Institute of Sciences, West Java, Indonesia.
出版信息
Vet World. 2021 Sep;14(9):2473-2481. doi: 10.14202/vetworld.2021.2473-2481. Epub 2021 Sep 23.
BACKGROUND AND AIM
Protamine (PRM) is the major protein in the sperm nucleus and plays an essential role in its normal function. Moreover, PRM has great potential as a protein marker of semen production and quality. This study aimed to assess the potential of sperm bovine PRM as a protein marker of semen production and quality in bulls at the National Artificial Insemination (AI) Center of Indonesia.
MATERIALS AND METHODS
The semen production capacity of each bull was collected from frozen semen production data at the Singosari AI Center for 6 months, and was then divided into two groups (high and low). A total of 440 frozen semen straws from six Limousin (LIM), six Friesian Holstein (FH), six Peranakan Ongole (PO), and four Aceh bulls aged 4-5 years were used in the study. The frozen semen was used to measure the concentration of PRM1, PRM2, and PRM3 using the enzyme immunoassay method. The frozen semen was also used to assess the quality of the semen, including progressive motility (PM) through computer-assisted semen analysis, sperm viability through eosin-nigrosin analysis, and the DNA fragmentation index through Acridine Orange staining.
RESULTS
PRM1 was significantly higher in all bull breeds included in the study (p<0.00), followed by PRM2 (p<0.00) and PRM3 (p<0.00). PRM1 significantly affected semen production in LIM, FH, PO, and Aceh bulls (p<0.05). Moreover, PRM2 significantly affected semen production only in FH and Aceh bulls (p<0.05), whereas PRM3 affected this parameter in PO and Aceh bulls exclusively (p<0.05). Consistently and significantly, PRM1 was positively correlated with the PM and viability of sperm and negatively associated with its DNA fragmentation in LIM, FH, PO, and Aceh bulls (p<0.05; p<0.01). The correlation analysis between PRM2 and PRM3 and semen quality parameters varied across all bull breeds; some were positively and negatively correlated (p<0.05; p<0.01), and some were not correlated at all.
CONCLUSION
PRM1 has excellent potential as a protein marker of semen production and quality in bulls at the National AI Center of Indonesia.
背景与目的
鱼精蛋白（PRM）是精子细胞核中的主要蛋白质，对其正常功能起着至关重要的作用。此外，PRM作为精液生成和质量的蛋白质标志物具有巨大潜力。本研究旨在评估印度尼西亚国家人工授精（AI）中心公牛精子PRM作为精液生成和质量蛋白质标志物的潜力。
材料与方法
从辛戈萨里AI中心6个月的冷冻精液生产数据中收集每头公牛的精液生产能力，然后分为两组（高和低）。本研究共使用了来自6头利木赞牛（LIM）、6头黑白花荷斯坦牛（FH）、6头佩拉纳坎翁戈尔牛（PO）和4头4 - 5岁亚齐牛的440支冷冻精液细管。使用酶免疫分析法测定冷冻精液中PRM1、PRM2和PRM3的浓度。冷冻精液还用于评估精液质量，包括通过计算机辅助精液分析测定的前向运动率（PM）、通过伊红 - 黑色素分析测定的精子活力以及通过吖啶橙染色测定的DNA碎片化指数。
结果
在本研究纳入的所有公牛品种中，PRM1显著更高（p<0.00），其次是PRM2（p<0.00）和PRM3（p<0.00）。PRM1对LIM、FH、PO和亚齐牛的精液生产有显著影响（p<0.05）。此外，PRM2仅对FH和亚齐牛的精液生产有显著影响（p<0.05），而PRM3仅对PO和亚齐牛的该参数有影响（p<0.05）。一致且显著的是，在LIM、FH、PO和亚齐牛中，PRM1与精子的PM和活力呈正相关，与DNA碎片化呈负相关（p<0.05；p<0.01）。PRM2和PRM3与精液质量参数之间的相关性分析在所有公牛品种中各不相同；有些呈正相关和负相关（p<0.05；p<0.01），有些则根本不相关。
结论
PRM1作为印度尼西亚国家AI中心公牛精液生产和质量的蛋白质标志物具有优异的潜力。
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