Morovský M, Pristas P, Javorský P, Nes I F, Holo H
Department of Biochemistry, Faculty of Science, P. J. Safárik University, Kosice, Slovakia.
Microbiol Res. 2001;156(2):133-8. doi: 10.1078/0944-5013-00090.
Enterocin BC25, a bacteriocin produced by Enterococcus faecium BC25 isolated from the rumen of cow was purified to homogeneity and sequenced. Twenty amino acids were identified in the peptide chain (TTHSGKYYGNGVYCT-KNKCT), identical to the N-terminal sequence of enterocin A. The DNA sequence of the enterocin BC25 structural gene and putative immunity protein exhibited high similarity to the entA gene. The occurrence of a 726 bp amplicon containing the enterocin A structural gene was studied among gram-positive ruminal cocci by PCR. Our results showed wide occurrence of the entA structural gene among ruminal enterococcal and streptococcal bacterial strains tested, and indicate variable ability to express bacteriocin production and resistance.
肠球菌素BC25是从奶牛瘤胃中分离出的粪肠球菌BC25产生的一种细菌素，已被纯化至同质并进行了测序。在肽链中鉴定出20个氨基酸（TTHSGKYYGNGVYCT-KNKCT），与肠球菌素A的N端序列相同。肠球菌素BC25结构基因和推定的免疫蛋白的DNA序列与entA基因具有高度相似性。通过PCR研究了革兰氏阳性瘤胃球菌中包含肠球菌素A结构基因的726 bp扩增子的出现情况。我们的结果表明，entA结构基因在所测试的瘤胃肠球菌和链球菌菌株中广泛存在，并表明表达细菌素产生和抗性的能力各不相同。
