亚硝基胍诱导粪肠球菌 MTCC 5153 突变株的分离与特性研究，该突变株在肠球菌素生物合成中存在缺陷。

Isolation and characterization of a nitrosoguanidine-induced Enterococcus faecium MTCC 5153 mutant defective in enterocin biosynthesis.

机构信息

Department of Food Microbiology, Central Food Technological Research Institute, Mysore 570 020, Council of Scientific and Industrial Research, India.

出版信息

Res Microbiol. 2010 Sep;161(7):590-4. doi: 10.1016/j.resmic.2010.05.010. Epub 2010 Jun 15.

DOI:10.1016/j.resmic.2010.05.010

PMID:20558282

Abstract

Enterocin A (EntA) is a low molecular weight, heat-stable, chromosomally encoded class IIa bacteriocin produced by several strains of Enterococcus faecium. In this study, a mutated strain of E. faecium MTCC 5153 was characterized for its sensitivity to EntA, immunity function and for the production of induction factor. Nucleotide sequencing of the putative promoter of the entA operon suggested point mutations upstream of the entA gene. The mutant was sensitive to several class IIa bacteriocins and was found to adsorb 20% more bacteriocin compared to its wild-type counterpart.

摘要

肠球菌素 A（EntA）是一种低分子量、热稳定的、染色体编码的 IIa 类细菌素，由几株屎肠球菌产生。在本研究中，对屎肠球菌 MTCC 5153 的突变株进行了特征描述，包括其对 EntA 的敏感性、免疫功能以及诱导因子的产生。EntA 操纵子的假定启动子的核苷酸序列表明，EntA 基因上游存在点突变。突变株对几种 IIa 类细菌素敏感，并且与野生型相比，其吸附的细菌素增加了 20%。