MacIntyre N
Edinburgh University Department of Veterinary Pathology, Easter Bush Veterinary Centre, Roslin, Midlothian, UK.
Br J Biomed Sci. 2001;58(3):190-6.
Preservation of tissue by fixation in a formalin solution, followed by dehydration and paraffin-wax embedding remains the predominant method of preparation for microscopic analysis of morphology. Whilst this may be optimal for morphological assessment, this technique has major disadvantages for subsequent immunohistochemical study as a result of the structural alteration of antigens that occurs during the processing procedure. However, the introduction of a range of antigen unmasking procedures has revolutionised immunohistochemistry, establishing the technique as a powerful tool in diagnostic pathology. Here, the development of enzymatic, non-enzymatic and heat-based antigen retrieval techniques is reviewed, and the methods in use currently are appraised.
通过在福尔马林溶液中固定组织，随后进行脱水和石蜡包埋，仍然是形态学微观分析的主要制备方法。虽然这可能是形态学评估的最佳方法，但由于在处理过程中抗原发生结构改变，该技术在后续免疫组织化学研究中存在重大缺点。然而，一系列抗原修复程序的引入彻底改变了免疫组织化学，使该技术成为诊断病理学中的强大工具。在此，对酶促、非酶促和基于热的抗原修复技术的发展进行综述，并对目前使用的方法进行评估。
