Shapiro B H, Goldman A S, Gustafsson J A
Endocrinology. 1975 Aug;97(2):487-92. doi: 10.1210/endo-97-2-487.
The hypothalamic-pituitary sex of the androgen insensitive, genetically male rat pseudohermaphrodite was studied by examining its vaginal cytology, response to ovarian transplants and urinary steroidal excretion patterns. More than half the pseudohermaphrodites studied were in constant vaginal estrus, while the remaining rats displayed either persistent diestrus or irregular cyclicity tending towards lengthened estrus. Following gonadectomy and ovarian transplantation, normal females displayed regular 14-day cycles while pseudohermaphrodites remained in constant vaginal estrus. In pseudohermaphrodites with ovarian transplants, only C19 steroids were detected in the urine while females excreted both C21 and C19 steroids. Indicative of the urinary findings, transplants in females had corpora lutea and maturing follicles while grafts from pseudohermaphrodites and males contained follicular cysts and luteinized theca. In addition, distribution and activity of histochemical 3beta-hydroxy-delta5 steroid oxidoreductase were similar in the grafts from pseudohermaphrodites and males, but unlike the females. Although previous reports have shown that much of the sex-dependent differentiation of the genetic male rat pseudohermaphrodite is phenotypically female, our results suggest that the phenotype of the hypothalamic-pituitary axis of this animal is, at least in certain respects, male.
通过检查雄激素不敏感的遗传性雄性大鼠假两性畸形的阴道细胞学、对卵巢移植的反应以及尿甾体排泄模式，对其下丘脑 - 垂体性别进行了研究。研究的假两性畸形大鼠中，超过一半处于持续的阴道发情期，而其余大鼠则表现为持续的动情间期或趋向于延长发情期的不规则周期。性腺切除和卵巢移植后，正常雌性表现出规律的14天周期，而假两性畸形大鼠则持续处于阴道发情期。在接受卵巢移植的假两性畸形大鼠中，尿液中仅检测到C19甾体，而雌性则同时排泄C21和C19甾体。与尿液检测结果一致，雌性的移植物中有黄体和成熟卵泡，而来自假两性畸形大鼠和雄性的移植物中含有卵泡囊肿和黄素化的膜细胞。此外，假两性畸形大鼠和雄性移植物中组织化学3β - 羟基 - Δ5甾体氧化还原酶的分布和活性相似，但与雌性不同。尽管先前的报道表明，遗传性雄性大鼠假两性畸形的许多性别依赖性分化在表型上是雌性的，但我们的结果表明，这种动物下丘脑 - 垂体轴的表型至少在某些方面是雄性的。
