Münscher A, Schmid M, Saeger W, Schreiber S, Lüdecke D K
Institute of Pathology, Marienkrankenhaus, Alfredstr. 9, D-22087 Hamburg, Germany.
Endocr Pathol. 2001 Summer;12(2):171-80. doi: 10.1385/ep:12:2:171.
In situ hybridization (ISH) enables the visualization of specific mRNA for pituitary hormones. Our collection consists of 40 surgically removed pituitary adenomas that were classified as follicle stimulating hormone/luteinizing hormone (FSH/LH) cell adenomas by structure and by immunostaining (IH) for all pituitary hormones. All forty adenomas were regarded as clinically inactive. The aim of our study was to examine nonfunctioning adenomas by ISH for demonstration of mRNAs for all pituitary hormones. The results were compared with proliferation markers, invasiveness and clinical data. ISH detected signals for all pituitary hormones at a range of 30% for prolactin (PRL) to 85% for proopiomelanocortin (POMC). mRNA for beta-FSH was detected in 70% and beta-LH mRNA in 43% of adenomas. Thirty-three percent of adenomas revealed negative mRNA detection for beta-LH but positive hormone content. The majority of adenomas (75%) expressed more than two mRNAs simultaneously, mostly the combination of POMC mRNA together with beta-FSH mRNA and one to four others. Comparison with clinical data showed no significant differences except for one adenoma with a high Ki-67 index (> 2.1% positive nuclei). This adenoma showed very high signals for PRL and beta-TSH mRNA.
原位杂交（ISH）可实现垂体激素特异性mRNA的可视化。我们的样本库包含40例手术切除的垂体腺瘤，这些腺瘤通过结构以及对所有垂体激素进行免疫染色（IH）被分类为促卵泡激素/促黄体生成素（FSH/LH）细胞腺瘤。所有40例腺瘤均被视为临床无功能型。我们研究的目的是通过ISH检查无功能腺瘤，以显示所有垂体激素的mRNA。将结果与增殖标志物、侵袭性及临床数据进行比较。ISH检测到所有垂体激素的信号，催乳素（PRL）为30%，阿黑皮素原（POMC）为85%。70%的腺瘤检测到β-FSH的mRNA，43%的腺瘤检测到β-LH的mRNA。33%的腺瘤β-LH的mRNA检测呈阴性，但激素含量呈阳性。大多数腺瘤（75%）同时表达两种以上的mRNA，主要是POMC mRNA与β-FSH mRNA以及一至四种其他mRNA的组合。与临床数据比较显示，除了一例Ki-67指数较高（>2.1%阳性细胞核）的腺瘤外，无显著差异。该腺瘤PRL和β-TSH mRNA显示出非常高的信号。
