Blann A D, Lanza F, Galajda P, Gurney D, Moog S, Cazenave J P, Lip G Y
University Department of Medicine, City Hospital, Birmingham, UK.
Thromb Haemost. 2001 Sep;86(3):777-83.
As platelet hyperactivity is important in atherosclerosis and smoking, we hypothesized higher levels of soluble platelet membrane glycoprotein V (gpV) in 95 patients with peripheral artery disease (PAD) and 92 with coronary artery disease (CAD) compared to 99 healthy controls, and examined the effects of aspirin and of smoking two cigarettes on soluble gpV and platelet function. Soluble gpV (ELISA) was significantly raised in, but not between, both PAD and CAD patients, compared to controls (p < 0.05). In multivariate analysis, systolic blood pressure, smoking and atherosclerosis (all p < 0.01) were significant influences on soluble gpV in the whole study cohort. There was a weak correlation between soluble gpV and another platelet marker, soluble P selectin (p = 0.048). Acute smoking in 14 subjects increased platelet aggregability and beta-thromboglobulin, but not soluble gpV: there were no changes in 11 non-smokers. Five days consumption of aspirin (325 mg daily) by 14 subjects did not influence levels of soluble gpV. Our data indicate that soluble gpV may be a useful new marker of platelet activation in atherosclerosis, but may be influenced by smoking status and blood pressure.
由于血小板活性亢进在动脉粥样硬化和吸烟过程中起着重要作用，我们推测95例外周动脉疾病（PAD）患者和92例冠状动脉疾病（CAD）患者的可溶性血小板膜糖蛋白V（gpV）水平高于99例健康对照者，并研究了阿司匹林和吸两支烟对可溶性gpV及血小板功能的影响。与对照组相比，可溶性gpV（酶联免疫吸附测定）在PAD和CAD患者中均显著升高，但在这两组患者之间无显著差异（p < 0.05）。在多变量分析中，收缩压、吸烟和动脉粥样硬化（所有p < 0.01）对整个研究队列中的可溶性gpV均有显著影响。可溶性gpV与另一种血小板标志物可溶性P选择素之间存在弱相关性（p = 0.048）。14名受试者急性吸烟后血小板聚集性和β-血小板球蛋白增加，但可溶性gpV未增加：11名不吸烟者无变化。14名受试者连续五天服用阿司匹林（每日325毫克）对可溶性gpV水平无影响。我们的数据表明，可溶性gpV可能是动脉粥样硬化中血小板活化的一种有用的新标志物，但可能受吸烟状况和血压的影响。
