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集胞藻同步培养物中的大分子合成

Macromolecular synthesis in synchronized cultures of Anacystis nidulans.

作者信息

Asato Y

出版信息

J Bacteriol. 1979 Oct;140(1):65-72. doi: 10.1128/jb.140.1.65-72.1979.

DOI:10.1128/jb.140.1.65-72.1979
PMID:115841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216780/
Abstract

Synchronous culture of Anacystis nidulans has been induced by the light-dark-light regimen. At various time intervals during synchronous growth, samples were pulsed with radioactive labels to determine phospholipid, protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA) syntheses within the cell division cycle. A temporal order of protein, RNA, and DNA syntheses occurred within the cell division cycle, whereas phospholipid was characteristically synthesized during midcycle (during cell enlargement) and during the time of cell division. Chemically determined protein, RNA, and DNA syntheses were found to support the schedule of these macromolecules in cultures growing at an 8-h doubling time.

摘要

通过明暗交替培养法诱导了集胞藻的同步培养。在同步生长的不同时间间隔，用放射性标记物对样品进行脉冲处理，以确定细胞分裂周期内磷脂、蛋白质、核糖核酸（RNA）和脱氧核糖核酸（DNA）的合成情况。在细胞分裂周期内，蛋白质、RNA和DNA的合成呈现出时间顺序，而磷脂的合成则典型地发生在周期中期（细胞增大期间）和细胞分裂时期。化学测定的蛋白质、RNA和DNA合成情况，被发现与在8小时倍增时间下生长的培养物中这些大分子的合成时间表相符。

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Macromolecular synthesis in synchronized cultures of Anacystis nidulans.集胞藻同步培养物中的大分子合成
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引用本文的文献

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Involvement of sulfoquinovosyl diacylglycerol in DNA synthesis in Synechocystis sp. PCC 6803.磺基喹喔啉基二酰基甘油参与集胞藻PCC 6803中的DNA合成。
BMC Res Notes. 2012 Feb 16;5:98. doi: 10.1186/1756-0500-5-98.
2
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J Bacteriol. 1983 Mar;153(3):1315-21. doi: 10.1128/jb.153.3.1315-1321.1983.
3
Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301.聚球藻属PCC 6301菌株中DNA循环与生长速率之间的关系。
J Bacteriol. 1990 May;172(5):2313-9. doi: 10.1128/jb.172.5.2313-2319.1990.
4
Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803: a blue-light-requiring process.集胞藻6803的光激活异养生长：一个需要蓝光的过程。
J Bacteriol. 1991 May;173(9):2761-7. doi: 10.1128/jb.173.9.2761-2767.1991.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates.大肠杆菌中脱氧核糖核酸合成的调控：对生长速率的依赖性。
Biochim Biophys Acta. 1966 Jun 22;119(3):517-25. doi: 10.1016/0005-2787(66)90128-6.
9
Observations on replication and cell division in synchronous cultures of the blue-green alga Anacystis nidulans.对蓝藻集胞藻6803同步培养物中复制和细胞分裂的观察。
J Bacteriol. 1971 Aug;107(2):583-4. doi: 10.1128/jb.107.2.583-584.1971.
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Biochemical studies on Anacystis nidulans during its synchronous growth.对集胞藻在同步生长期间的生化研究。
Arch Mikrobiol. 1969;69(1):34-9. doi: 10.1007/BF00408560.