集胞藻同步培养物中的大分子合成

Macromolecular synthesis in synchronized cultures of Anacystis nidulans.

作者信息

Asato Y

出版信息

J Bacteriol. 1979 Oct;140(1):65-72. doi: 10.1128/jb.140.1.65-72.1979.

DOI:10.1128/jb.140.1.65-72.1979

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216780/

Abstract

Synchronous culture of Anacystis nidulans has been induced by the light-dark-light regimen. At various time intervals during synchronous growth, samples were pulsed with radioactive labels to determine phospholipid, protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA) syntheses within the cell division cycle. A temporal order of protein, RNA, and DNA syntheses occurred within the cell division cycle, whereas phospholipid was characteristically synthesized during midcycle (during cell enlargement) and during the time of cell division. Chemically determined protein, RNA, and DNA syntheses were found to support the schedule of these macromolecules in cultures growing at an 8-h doubling time.

摘要

通过明暗交替培养法诱导了集胞藻的同步培养。在同步生长的不同时间间隔，用放射性标记物对样品进行脉冲处理，以确定细胞分裂周期内磷脂、蛋白质、核糖核酸（RNA）和脱氧核糖核酸（DNA）的合成情况。在细胞分裂周期内，蛋白质、RNA和DNA的合成呈现出时间顺序，而磷脂的合成则典型地发生在周期中期（细胞增大期间）和细胞分裂时期。化学测定的蛋白质、RNA和DNA合成情况，被发现与在8小时倍增时间下生长的培养物中这些大分子的合成时间表相符。

相似文献

1

Macromolecular synthesis in synchronized cultures of Anacystis nidulans.

J Bacteriol. 1979 Oct;140(1):65-72. doi: 10.1128/jb.140.1.65-72.1979.

2

Biochemical studies on Anacystis nidulans during its synchronous growth.

Arch Mikrobiol. 1969;69(1):34-9. doi: 10.1007/BF00408560.

3

Temporal genetic mapping of the blue-green alga, Anacystis nidulans.

Genetics. 1970 Jul;65(3):407-19. doi: 10.1093/genetics/65.3.407.

4

Observations on replication and cell division in synchronous cultures of the blue-green alga Anacystis nidulans.

J Bacteriol. 1971 Aug;107(2):583-4. doi: 10.1128/jb.107.2.583-584.1971.

5

Dark incubation causes reinitiation of cell cycle events in Anacystis nidulans.

J Bacteriol. 1983 Mar;153(3):1315-21. doi: 10.1128/jb.153.3.1315-1321.1983.

6

Toward an understanding of cell growth and the cell division cycle of unicellular photoautotrophic cyanobacteria.

Cell Mol Life Sci. 2003 Apr;60(4):663-87. doi: 10.1007/s00018-003-2079-y.

7

Drift in ultraviolet sensitivity and expression of mutations during synchronous growth of cyanobacterium Anacystis nidulans.

Mutat Res. 1983 Feb;107(2):229-38. doi: 10.1016/0027-5107(83)90165-3.

8

The regulation of stable RNA synthesis in the blue-green alga Anacystis nidulans: effect of leucine deprivation and 5-methyltryptophan inhibition.

J Gen Microbiol. 1977 Nov;103(1):61-8. doi: 10.1099/00221287-103-1-61.

9

Mutational analysis of dark endogenous metabolism in the blue-green bacterium Anacystis nidulans.

J Bacteriol. 1974 Sep;119(3):677-83. doi: 10.1128/jb.119.3.677-683.1974.

10

The DNA, RNA and protein composition of the cyanobacterium Anacystis nidulans grown in light- and carbon dioxide-limited chemostats.

Arch Microbiol. 1980 Aug;127(1):53-8. doi: 10.1007/BF00414355.

引用本文的文献

1

Involvement of sulfoquinovosyl diacylglycerol in DNA synthesis in Synechocystis sp. PCC 6803.

BMC Res Notes. 2012 Feb 16;5:98. doi: 10.1186/1756-0500-5-98.

2

Dark incubation causes reinitiation of cell cycle events in Anacystis nidulans.

J Bacteriol. 1983 Mar;153(3):1315-21. doi: 10.1128/jb.153.3.1315-1321.1983.

3

Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301.

J Bacteriol. 1990 May;172(5):2313-9. doi: 10.1128/jb.172.5.2313-2319.1990.

4

Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803: a blue-light-requiring process.

J Bacteriol. 1991 May;173(9):2761-7. doi: 10.1128/jb.173.9.2761-2767.1991.

本文引用的文献

1

Protein measurement with the Folin phenol reagent.

J Biol Chem. 1951 Nov;193(1):265-75.

2

Determination of nucleic acids in animal tissues.

J Biol Chem. 1955 May;214(1):59-70.

3

An ultramicro technique for the determination of deoxypentose nucleic acid.

Arch Biochem Biophys. 1956 Aug;63(2):446-51. doi: 10.1016/0003-9861(56)90059-5.

4

Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardi. I. Isotopic transfer experiments with a strain producing eight zoospores.

J Mol Biol. 1967 Apr 14;25(1):47-66. doi: 10.1016/0022-2836(67)90278-1.

5

Ultrastructure of the cell wall and cell division of unicellular blue-green algae.

J Bacteriol. 1968 Sep;96(3):842-52. doi: 10.1128/jb.96.3.842-852.1968.

6

DNA cycle of Myxococcus xanthus.

J Mol Biol. 1970 May 14;49(3):609-19. doi: 10.1016/0022-2836(70)90285-8.

7

Onset of DNA synthesis during the cell cycle in chemostat cultures.

Proc Natl Acad Sci U S A. 1967 Jun;57(6):1611-7. doi: 10.1073/pnas.57.6.1611.

8

Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates.

Biochim Biophys Acta. 1966 Jun 22;119(3):517-25. doi: 10.1016/0005-2787(66)90128-6.

9

Observations on replication and cell division in synchronous cultures of the blue-green alga Anacystis nidulans.

J Bacteriol. 1971 Aug;107(2):583-4. doi: 10.1128/jb.107.2.583-584.1971.

10

Biochemical studies on Anacystis nidulans during its synchronous growth.

Arch Mikrobiol. 1969;69(1):34-9. doi: 10.1007/BF00408560.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。