Asato Y
J Bacteriol. 1983 Mar;153(3):1315-21. doi: 10.1128/jb.153.3.1315-1321.1983.
Synchronized cultures of Anacystis nidulans (Synechococcus PCC 6301), an obligate phototroph, are obtained by incubating exponential cultures in the dark for 12 to 16 h. A temporal and sequential order of macromolecular synthesis is observed within the cell division cycle of a synchronously dividing culture in the light. Apparently, dark incubation causes the cells to realign their cellular activities in such a way that all cells emerge from the dark and grow synchronously in the light. A study was conducted to explore the possible mechanisms responsible for the putative dark-induction process. Samples were taken at various times from a synchronized culture and were subjected to another round of dark incubation for 16 h. When these cultures were returned to the light, the cell number increased from 3 h and doubled at about 7 h. The protein, RNA, and DNA contents started to increase in order well before 3 h. This general pattern of cellular activities, observed for nearly all samples (i.e., for cells of different physiological ages), indicated that the dark incubation period caused the ongoing cell cycle to abort and a new cell cycle to be reinitiated under light growth conditions.
通过将处于指数生长期的培养物在黑暗中孵育12至16小时，可获得专性光合生物——集胞藻（聚球藻PCC 6301）的同步培养物。在光照条件下同步分裂培养物的细胞分裂周期内，可观察到大分子合成的时间和顺序。显然，黑暗孵育使细胞重新调整其细胞活动，使得所有细胞从黑暗中出来并在光照下同步生长。开展了一项研究以探索导致假定的黑暗诱导过程的可能机制。从同步培养物中在不同时间取样，并进行另一轮16小时的黑暗孵育。当这些培养物重新置于光照下时，细胞数量在3小时开始增加，并在约7小时翻倍。蛋白质、RNA和DNA含量早在3小时之前就开始依次增加。几乎所有样品（即不同生理年龄的细胞）观察到的这种细胞活动的一般模式表明，黑暗孵育期导致正在进行的细胞周期中止，并在光照生长条件下重新启动一个新的细胞周期。
