一种利用固相萃取柱中9-芴甲基氯甲酸酯进行柱前衍生化及反相高效液相色谱法测定人血浆中黏菌素的简单方法。
A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography.
作者信息
Li J, Milne R W, Nation R L, Turnidge J D, Coulthard K, Johnson D W
机构信息
Centre for Pharmaceutical Research, University of South Australia, Adelaide Australia.
出版信息
J Chromatogr B Biomed Sci Appl. 2001 Sep 25;761(2):167-75. doi: 10.1016/s0378-4347(01)00326-7.
A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%.
描述了一种简单、选择性和灵敏的高效液相色谱（HPLC）方法，用于测定人血浆中的多粘菌素。用9-芴基甲基氯甲酸酯进行衍生化反应，在用于样品预处理的同一固相萃取C18柱中进行，然后进行反相HPLC并采用荧光检测。通过多粘菌素A和B衍生物的总峰面积与奈替米星（内标）衍生物的峰面积之比进行定量。在血浆中硫酸多粘菌素浓度为0.10至4.0mg/l范围内获得线性校准曲线。准确度在10%以内，重现性（相对标准偏差）小于10%。
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