Raghuraman M K, Winzeler E A, Collingwood D, Hunt S, Wodicka L, Conway A, Lockhart D J, Davis R W, Brewer B J, Fangman W L
Department of Genetics, Department of Mathematics, University of Washington, Seattle, WA 98195, USA.
Science. 2001 Oct 5;294(5540):115-21. doi: 10.1126/science.294.5540.115.
Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae. The times of replication of thousands of sites across the genome were determined by hybridizing replicated and unreplicated DNAs, isolated at different times in S phase, to the microarrays. Origin activations take place continuously throughout S phase but with most firings near mid-S phase. Rates of replication fork movement vary greatly from region to region in the genome. The two ends of each of the 16 chromosomes are highly correlated in their times of replication. This microarray approach is readily applicable to other organisms, including humans.
寡核苷酸微阵列被用于绘制出芽酵母酿酒酵母中染色体复制的详细拓扑结构。通过将在S期不同时间分离的已复制和未复制DNA与微阵列杂交，确定了全基因组数千个位点的复制时间。起始点激活在整个S期持续发生，但大多数起始发生在S期中期附近。复制叉移动速率在基因组的不同区域差异很大。16条染色体中每条染色体的两端在复制时间上高度相关。这种微阵列方法很容易应用于包括人类在内的其他生物体。
