Afanas'ev I B, Ostrakhovitch E A, Mikhal'chik E V, Korkina L G
Vitamin Research Institute, Nauchny pr. 14A, Moscow 117820, Russia.
Luminescence. 2001 Sep-Oct;16(5):305-7. doi: 10.1002/bio.659.
The interaction of superoxide ion with lucigenin produces chemiluminescence (CL), which is widely used for the detection of this radical anion. However, in many biological systems lucigenin may be directly reduced to its semiquinone by some enzymes. We found that if the direct reduction of lucigenin takes place, it decreases superoxide production due to the competition with one-electron reduction of dioxygen to superoxide ion. Comparison of two methods of superoxide detection (lucigenin-amplified CL and cytochrome c reduction) showed that there are excellent correlations between the results obtained by the two methods. Hence, lucigenin-amplified CL remains a sensitive and reliable assay of superoxide detection.
超氧阴离子与光泽精相互作用会产生化学发光（CL），这被广泛用于检测这种自由基阴离子。然而，在许多生物系统中，光泽精可能会被某些酶直接还原为其半醌。我们发现，如果发生光泽精的直接还原，由于与将氧气单电子还原为超氧阴离子的竞争，它会减少超氧的产生。两种超氧检测方法（光泽精放大化学发光法和细胞色素c还原法）的比较表明，两种方法获得的结果之间存在极好的相关性。因此，光泽精放大化学发光法仍然是一种灵敏且可靠的超氧检测方法。
