Liochev S I, Fridovich I
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Free Radic Biol Med. 1998 Nov 15;25(8):926-8. doi: 10.1016/s0891-5849(98)00121-x.
Lucigenin caused a concentration-dependent increase in superoxide production by xanthine oxidase plus xanthine. This was seen, in terms of superoxide dismutase-inhibitable reduction of cytochrome c; in spite of the ability of univalently reduced lucigenin to directly reduce cytochrome c. It follows that in the absence of this interference, by the cytochrome, an even greater increase in superoxide production mediated by lucigenin would have been observed. Clearly lucigenin luminescence should not be relied upon as a method for measurement of, or even for detection of, superoxide.
光泽精导致黄嘌呤氧化酶加黄嘌呤产生超氧化物的量呈浓度依赖性增加。这一点可通过超氧化物歧化酶可抑制的细胞色素c还原看出；尽管单价还原的光泽精有直接还原细胞色素c的能力。由此可见，在没有细胞色素这种干扰的情况下，会观察到光泽精介导的超氧化物产生量有更大的增加。显然，不应将光泽精发光作为测量甚至检测超氧化物的方法。
