Jaffe E A, Nachman R L
J Clin Invest. 1975 Sep;56(3):698-702. doi: 10.1172/JCI108140.
Cultured human endothelial cells were labeled with (3H)leucine, and the radioactive Factor VIII antigen present in the postculture medium was isolated by double anitbody immunoprecipitation and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol. The Factor VIII antigen synthesized by cultured endothelial cells was found to contain the same single polypeptide subunit (mol wt 225,000) present in plasma Factor VIII antigen. These results suggest that in vivo, the endothelial cell is a major site of synthesis of circulating Factor VIII antigen.
将培养的人内皮细胞用(3H)亮氨酸标记,然后通过双抗体免疫沉淀法分离培养后培养基中存在的放射性因子VIII抗原,并在使用二硫苏糖醇还原后通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行表征。发现培养的内皮细胞合成的因子VIII抗原含有与血浆因子VIII抗原中相同的单一多肽亚基(分子量225,000)。这些结果表明,在体内,内皮细胞是循环因子VIII抗原的主要合成部位。