Zhang G, Zhao Z, Xu S, Ni L, Wang X
Department of Gastroenterology, First Affiliated Hospital, Nanjing Medical University, 300 Guang-Zhou-Road, Nanjing 210029, Jiangsu Province, China.
Chin Med J (Engl). 1999 Apr;112(4):330-2.
To investigate the expression of autotaxin (ATX) mRNA existed in the human hepatocellular carcinoma (HCC), and whether there is relation between the level of ATX expression and clinicopathological features of HCC.
Five normal liver tissues and 32 histologically verified HCC specimens were obtained. Semi-quantitative reverse transcription polymerase chain reaction was used to detect mRNA expression of ATX.
ATX was expressed in all 32 HCC and 5 normal liver tissues. The mean expression level of ATX gene in HCC samples was higher than that in normal liver tissues (68.23% +/- 15.31% vs. 31.97% +/- 8.05%, P < 0.001). Patients were divided into two groups: low ATX HCC (15 cases) and high ATX HCC (17 cases) by the cutoff point of median value. Intrahepatic metastasis, vascular invasion and poor differentiation were more frequently noted in HCC patients with high ATX expression than in patients with low ATX expression.
ATX gene was found to be overexpressed in some HCC and correlated with HCC development and metastases.
研究自分泌运动因子（ATX）mRNA在人肝细胞癌（HCC）中的表达情况，以及ATX表达水平与HCC临床病理特征之间是否存在关联。
获取5例正常肝组织和32例经组织学证实的HCC标本。采用半定量逆转录聚合酶链反应检测ATX的mRNA表达。
32例HCC和5例正常肝组织均表达ATX。HCC样本中ATX基因的平均表达水平高于正常肝组织（68.23%±15.31%对31.97%±8.05%，P<0.001）。以中位数为界值将患者分为两组：低ATX HCC组（15例）和高ATX HCC组（17例）。ATX高表达的HCC患者肝内转移、血管侵犯及低分化情况比ATX低表达患者更常见。
发现ATX基因在部分HCC中过度表达，且与HCC的发生发展及转移相关。
