Ryan T A
Department of Biochemistry, The Weill Medical College of Cornell University, New York, NY 10021, USA.
Curr Opin Neurobiol. 2001 Oct;11(5):544-9. doi: 10.1016/s0959-4388(00)00247-6.
Understanding the detailed molecular events that support chemical synaptic transmission requires high-resolution methods that provide quantitative information combined with molecular specificity. In recent years, many new technological approaches, including genetically encoded fluorescent indicators, ultra-thin sectioning, and live-cell imaging have been brought to bear on understanding the cell biology and physiology of presynaptic terminals.
要了解支持化学突触传递的详细分子事件，需要高分辨率方法，这些方法能提供定量信息并兼具分子特异性。近年来，许多新技术方法，包括基因编码荧光指示剂、超薄切片和活细胞成像，都已被用于理解突触前终末的细胞生物学和生理学。
