Imanaka Y, Takeuchi H
Department of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
Chem Senses. 2001 Oct;26(8):1023-7. doi: 10.1093/chemse/26.8.1023.
The whole-cell, patch clamp [corrected] method was applied to olfactory receptor cells in slice preparations made from bullfrog olfactory epithelium. Under voltage-clamp conditions, olfactory receptor cells showed a transient inward current followed by a steady outward current in response to depolarizing voltage steps, as has been shown in the isolated preparation. The input resistance was 5.4 +/- 3.9 GOmega and capacitance 21.9 +/- 9.7 pF. Under current-clamp conditions, depolarization of cells by current injection induced action potentials. In 13 out of 20, spike generation was repetitive with a maximum frequency of 24 Hz. The frequency of the repetitive discharges increased as the injected current was increased. The relationship between the size of the injected current and firing frequency could be well fitted by the Michaelis-Menten equation, indicating that the spike generation site lacks the non-linear boosting system. The slice preparation developed here would provide a powerful tool to study the spike encoding system of the olfactory receptor cells.
全细胞膜片钳[校正后]方法应用于由牛蛙嗅上皮制备的切片中的嗅觉受体细胞。在电压钳条件下，嗅觉受体细胞在去极化电压阶跃刺激下表现出一个短暂的内向电流，随后是一个稳定的外向电流，这与在分离制备物中所显示的情况相同。输入电阻为5.4±3.9 GΩ，电容为21.9±9.7 pF。在电流钳条件下，通过电流注入使细胞去极化可诱发动作电位。在20个细胞中有13个，动作电位的产生是重复性的，最大频率为24 Hz。重复放电的频率随着注入电流的增加而增加。注入电流大小与放电频率之间的关系可以很好地用米氏方程拟合，这表明动作电位产生部位缺乏非线性增强系统。这里开发的切片制备将为研究嗅觉受体细胞的动作电位编码系统提供一个强大的工具。
