Pyhälä R, Ikonen N, Santanen R, Haanpää M, Visakorpi R, Jäppinen P, Valle M
National Public Health Institute, Mannerheimintie 166, 00300 Helsinki, Finland.
J Med Virol. 2001 Nov;65(3):584-9.
Intraepidemic antigenic and genetic variation was indicated when H3N2-subtype influenza A virus strains isolated during the 1996-1997 epidemic season in Finland were studied for reactivity in the haemagglutination inhibition (HI) assay and for nucleotide sequences coding for the variable HA1 domain of viral haemagglutinin. Thirty prevaccination- and postvaccination-paired sera taken from subjects who had been vaccinated against influenza during the previous autumn were studied for the presence of HI antibody to the homologous vaccine virus A/Nanchang/933/95, and five field strains representing the genetic and antigenic variability of the 1996-1997 epidemic season. The lowest vaccination-induced HI titres in each of the three age groups were detected in the two field strains that had been isolated from vaccinated patients and belonged to two different genetic sublineages. The intraepidemic variability of the 1996-1997 field strains in HI reactivity may be indicative of circulation of virus strains that may be capable of breaking through vaccination-induced immunity better than the other strains.
对在芬兰1996 - 1997流行季节分离出的H3N2亚型甲型流感病毒株进行血凝抑制（HI）试验反应性及编码病毒血凝素可变HA1结构域的核苷酸序列研究时，发现了流行期间的抗原性和基因变异。研究了从去年秋季接种流感疫苗的受试者采集的30份接种前和接种后配对血清，检测其针对同源疫苗病毒A/南昌/933/95的HI抗体，以及代表1996 - 1997流行季节基因和抗原变异性的5株现场分离株。在从接种疫苗患者中分离出且属于两个不同基因亚系的两株现场分离株中，检测到三个年龄组中每组最低的疫苗诱导HI滴度。1996 - 1997年现场分离株在HI反应性方面的流行期间变异性可能表明，某些病毒株的传播可能比其他病毒株更能突破疫苗诱导的免疫力。
