Li X J, Yu H M, Koide S S
Department of Pharmacology, Beijing Medical University, Beijing 100083.
Yao Xue Xue Bao. 1997 Aug;32(8):586-92.
In the present studies, we observed the regulation of water channel gene (AQP-CHIP) expression by estradiol (E2) and anordiol, an antiestrogen with agonist activity, in immature female rat uterus. Antisense and sense oligonucleotide primers corresponding to the consensus sequences of two rats AQP-CHIP water channels were synthesized and used to amplify a cDNA fragment that was reverse-transcripted from rat uterine total RNA preparation. E2 administered as a single dose of 40 micrograms.kg-1 to immature female rats induced a significant increase in AQP-CHIP mRNA expression 9 h after treatment. The lowest effective doses of E2 and anordiol were 40 and 50 micrograms.kg-1, respectively. The stimulatory effect of anordiol was more pronounced than that of E2. The present results suggest that AQP-CHIP water channel gene expression may be involved in E2- and anordiol-mediated water imbibition and luminal fluid production in the uterus.
在本研究中，我们观察了雌二醇（E2）和具有激动剂活性的抗雌激素药物炔诺醇对未成熟雌性大鼠子宫水通道基因（AQP-CHIP）表达的调控。合成了与两种大鼠AQP-CHIP水通道共有序列相对应的反义及正义寡核苷酸引物，并用于扩增从大鼠子宫总RNA制备物逆转录得到的cDNA片段。以40微克·千克-1的单剂量给未成熟雌性大鼠注射E2，处理9小时后诱导AQP-CHIP mRNA表达显著增加。E2和炔诺醇的最低有效剂量分别为40和50微克·千克-1。炔诺醇的刺激作用比E2更明显。目前的结果表明，AQP-CHIP水通道基因表达可能参与E2和炔诺醇介导的子宫水吸收及管腔液生成。
