高效液相色谱法测定银杏叶中六种黄酮类成分

[HPLC determination of six flavonoid constituents in Ginkgo biloba leaves].

作者信息

Chi J D, He X F, Liu A R, Xu L X

机构信息

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050.

出版信息

Yao Xue Xue Bao. 1997 Aug;32(8):625-8.

PMID:11596315

Abstract

Six flavonoid constituents (quercetin, isorhamnetin, kaempferol, bilobetin, ginkgetin and sciadopitysin) were isolated from Ginkgo biloba leaves and determined by reversed phase HPLC using salvianolic acid B as internal standard. The column employed was Zorbax ODS (150 mm x 4 mm ID, 5 microns). The mobile phase consisted of solvent A (methanol) and solvent B [tetrahydrofuran--water--formic acid (34:65:1)] for gradient elution. The flow rate was 1 ml.min-1 and detection was effected at 350 nm. This method is accurate, rapid and reproducible. Analytical data for various samples were given.

摘要

从银杏叶中分离出六种黄酮类成分（槲皮素、异鼠李素、山奈酚、白果双黄酮、银杏黄素和金钱松双黄酮），并以丹酚酸B为内标，采用反相高效液相色谱法进行测定。所用色谱柱为Zorbax ODS（150 mm×4 mm内径，5微米）。流动相由溶剂A（甲醇）和溶剂B[四氢呋喃-水-甲酸（34:65:1）]组成，用于梯度洗脱。流速为1 ml·min-1，检测波长为350 nm。该方法准确、快速且具有重现性。给出了各种样品的分析数据。