Zhao Yi-Yi, Wang Jing-Hui, Fu Xin-Tong, Chen You-Gen, Guo Hong-Zhu
Beijing Institute for Drug Control, Beijing 100035, China.
Yao Xue Xue Bao. 2013 Jan;48(1):98-103.
A new UPLC method was developed for the simultaneous determination of eleven characteristic flavonoid glycosides in Ginkgo biloba leaves. The natural occurrence of flavonoid glycosides in Ginkgo biloba leaves within one vegetative season was investigated for the first time. The analysis was performed on an Agilent ZORBAX Eclipse Plus C18 column (50 mm x 4.6 mm, 1.8 microm), the mobile phase A was acetonitrile, the mobile phase B was 0.4% phosphate aqueous solution in a gradient elution at a flow rate of 0.6 mL x min(-1), the detection was carried out at 360 nm. The result showed that eleven flavonoid glycosides had good linearity with good average recovery, separately. The method was proved to be accurate, rapid and good reproducible for the quality evaluation of Ginkgo biloba leaves, and provide an easy and rapid means for the quantitative analysis of flavonoid glycosides and their content fluctuation with seasons.
建立了一种超高效液相色谱法同时测定银杏叶中11种特征黄酮苷。首次对一个生长季内银杏叶中黄酮苷的自然存在情况进行了研究。分析采用安捷伦ZORBAX Eclipse Plus C18柱（50 mm×4.6 mm，1.8 µm），流动相A为乙腈，流动相B为0.4%磷酸水溶液，梯度洗脱，流速为0.6 mL·min⁻¹，检测波长为360 nm。结果表明，11种黄酮苷线性关系良好，平均回收率良好。该方法被证明对银杏叶质量评价准确、快速且重现性好，为黄酮苷的定量分析及其随季节的含量波动提供了一种简便快速的方法。
