Lv Jin-Li, Yang Biao, Li Meng-Xuan, Meng Zhao-Qing, Ma Shi-Ping, Wang Zhen-Zhong, Ding Gang, Huang Wen-Zhe, Xiao Wei
Jiang Su Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
China Pharmaceutical university, Nanjing 210009, China.
Zhongguo Zhong Yao Za Zhi. 2017 Mar;42(5):931-935. doi: 10.19540/j.cnki.cjcmm.2017.0028.
To study Ginkgo biloba leaves in different producing area, we establish an HPLC method for the simultaneously determination of seven flavonoids glycosides and four biflavonoids in G. biloba leaves. The analysis was performed on an Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm) wich acetonitrile, and 0.4% phosphoric acid as mobile phase at flow rate of 1 mL•min⁻¹ in a gradient edution, and the detection was carried out at 254 nm.The calibration curves of the seven flavonoids glycosides and four biflavonoids had a good linearitiy with good recoveries. The established HPLC method is simple, rapid, accurate, reliable, and sensitive, and can be applied to the identification and quality control of G. biloba leaves.
为研究不同产地的银杏叶，我们建立了一种高效液相色谱法，用于同时测定银杏叶中七种黄酮苷和四种双黄酮。分析在安捷伦ZORBAX SB-C₁₈柱（4.6 mm×250 mm，5μm）上进行，以乙腈和0.4%磷酸为流动相，流速为1 mL•min⁻¹，采用梯度洗脱，检测波长为254 nm。七种黄酮苷和四种双黄酮的校准曲线线性良好，回收率高。所建立的高效液相色谱法简便、快速、准确、可靠、灵敏，可用于银杏叶的鉴别和质量控制。
