Nedukha O M, Vasyltsov I V
Department of Cell Biology and Anatomy, Institute of Botany, Kiev, Ukraine.
Adv Space Res. 2001;27(5):977-81. doi: 10.1016/s0273-1177(01)00185-5.
Formation and structure of potato minitubers grown aseptically for 30 days on a horizontal clinostat and in stationary control have been studied by light and electron microscopy. It was demonstrated that the number of plants that formed minitubers, their size and fresh weight, was higher when clino-rotated than in the stationary control. It was revealed that the amount of amyloplasts in parenchyma cell sections was doubled in minitubers formed under clino-rotation. Other factors (shape of minitubers and size of reserve parenchyma cells) did not differ from the stationary control. The changes in amyloplast ultrastructure suggest accelerated cell maturity of potato reserve parenchyma in extended clino-rotation.
利用光学显微镜和电子显微镜,研究了在水平回转器上无菌培养30天的马铃薯微型薯块以及静止对照中微型薯块的形成和结构。结果表明,与静止对照相比,进行回转处理时形成微型薯块的植株数量、微型薯块的大小和鲜重均更高。研究发现,在回转处理下形成的微型薯块中,薄壁细胞切片中的造粉体数量增加了一倍。其他因素(微型薯块的形状和贮藏薄壁细胞的大小)与静止对照没有差异。造粉体超微结构的变化表明,延长回转处理会加速马铃薯贮藏薄壁细胞的成熟。