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芦荟渗出液:采用反相高效液相色谱法和顶空气相色谱 - 质谱联用进行表征

Aloe exudate: characterization by reversed phase HPLC and headspace GC-MS.

作者信息

Saccù D, Bogoni P, Procida G

机构信息

Dipartimento di Economia e Merceologia delle Risorse Naturali e della Produzione, Via A. Valerio 6, I-34127 Trieste, Italy.

出版信息

J Agric Food Chem. 2001 Oct;49(10):4526-30. doi: 10.1021/jf010179c.

DOI:10.1021/jf010179c
PMID:11599983
Abstract

From the leaves of aloe, a succulent plant, a dried exudate commonly called aloe can be obtained, which is used as a natural drug for its cathartic effect and is widely employed as a bittering agent in alcoholic beverages. This investigation provides a tentative characterization of several commercial aloe exudates carried out both by reversed phase HPLC and by headspace GC-MS analysis. By means of HPLC the derivatives were evaluated, and by GC-MS the volatile fraction was investigated. Qualitative and quantitative differences among the constituents in various samples of different origins were found. In particular, these were evident in the HPLC profile of Kenya aloe and an Aloe barbadensis sample, which exuded a high content of isoaloeresin D and aloins, whereas GC-MS analysis showed the presence of anisole exclusively in Kenya aloe samples. Moreover, the results obtained by means of the latter technique suggested a reason for the prevailing use of Mosselbay and Port Elizabeth aloes in bitter spirits formulation.

摘要

从肉质植物芦荟的叶子中,可以获得一种通常称为芦荟的干燥渗出物,它因其通便作用而被用作天然药物,并广泛用作酒精饮料中的苦味剂。本研究通过反相高效液相色谱法(reversed phase HPLC)和顶空气相色谱 - 质谱联用分析法(headspace GC-MS)对几种商业芦荟渗出物进行了初步表征。通过高效液相色谱法评估衍生物,通过气相色谱 - 质谱联用分析法研究挥发性成分。发现不同来源的各种样品中成分存在定性和定量差异。特别是在肯尼亚芦荟和库拉索芦荟样品的高效液相色谱图中很明显,它们渗出物中异芦荟树脂D和芦荟苷含量很高,而气相色谱 - 质谱联用分析表明仅在肯尼亚芦荟样品中存在苯甲醚。此外,后一种技术获得的结果为莫塞尔湾芦荟和伊丽莎白港芦荟在苦酒配方中普遍使用提供了一个原因。

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