Dai J, Yaylayan V A, Raghavan G S, Paré J R, Liu Z, Bélanger J M
Department of Food Science and Agricultural Chemistry and Department of Agricultural and Biosystems Engineering, Macdonald Campus of McGill University, 21,111 Lakeshore, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada.
J Agric Food Chem. 2001 Oct;49(10):4584-8. doi: 10.1021/jf010592k.
The use of the microwave-assisted process (MAP) for the extraction of azadirachtin-related limonoids (AZRL) from various parts of the neem tree was investigated under different operating conditions. The influence of microwave power, solvent, and irradiation time on the recovery of AZRL was studied. The efficiency of the microwave-assisted extraction (MAE) of the seed kernel, the seed shell, the leaf, and the leaf stem was compared to that of conventional extraction methods. The content of AZRL in the extracts was estimated with a vanillin-based colorimetric assay and a multivariate calibration technique. The results showed that the MAE technique can enhance the extraction of AZRL from different parts of neem possessing microstructures. Investigation of the influence of the solvent also indicted that the solvent used not only influences the efficiency but also affects the selectivity of the MAE.
研究了在不同操作条件下，利用微波辅助工艺（MAP）从印楝树的各个部位提取印楝素相关柠檬苦素（AZRL）。研究了微波功率、溶剂和辐照时间对AZRL回收率的影响。将种子内核、种子外壳、树叶和叶柄的微波辅助萃取（MAE）效率与传统萃取方法进行了比较。采用基于香草醛的比色法和多元校准技术对提取物中AZRL的含量进行了估算。结果表明，MAE技术可提高从具有微观结构的印楝不同部位提取AZRL的效率。对溶剂影响的研究还表明，所使用的溶剂不仅影响效率，而且还影响MAE的选择性。
