Ambrosino P, Fresa R, Fogliano V, Monti S M, Ritieni A
Dipartimento di Scienza degli Alimenti, Università di Napoli "Federico II" Parco Gussone, 80055 Portici (Napoli), Italy.
J Agric Food Chem. 1999 Dec;47(12):5252-6. doi: 10.1021/jf9905368.
A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO(2)) were used as extractive agents in a three-separation-stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.
一种新的超临界萃取方法被应用于从印楝种仁中提取印楝素A（AZA - A）。在一个三级分离阶段的超临界中试装置中，超临界和液态二氧化碳（CO₂）被用作萃取剂。也对亚临界条件进行了测试。通过计算中试装置相对于每千克种子所提取的AZA - A的毫克数（ms/mo）的效率来进行比较。使用119而非64的ms/mo比率获得了最便捷的萃取效果。对于超临界萃取，在中试装置中建立了从油中分离表皮蜡质的方法。采用高效液相色谱法（HPLC）和电喷雾质谱法来监测AZA - A的萃取产率。
