Mateus N, Silva A M, Vercauteren J, de Freitas V
Centro de Investigação em Química, Departamento de Química do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal.
J Agric Food Chem. 2001 Oct;49(10):4836-40. doi: 10.1021/jf001505b.
Several anthocyanin-derived pigments that showed UV-visible spectra different from those of the original grape anthocyanins were detected by HPLC-DAD analysis in 1-year-old bottled Port wines from the Douro region. Among these, three malvidin 3-glucoside derived pigments were detected in large amounts, representing approximately 60% of the total anthocyanidin monoglucosides content. These pigments were isolated, purified, and identified by LSI-MS and NMR ((1)H, DQF-COSY, ROESY, HSQC, and HMBC) techniques. The major pigment is malvidin 3-glucoside pyruvic adduct, previously characterized, and the other two corresponded to its respective acetyl and coumaroyl glucoside derivatives. The latter is reported for the first time in red wines.
通过高效液相色谱-二极管阵列检测（HPLC-DAD）分析，在杜罗河地区1年陈酿的瓶装波特葡萄酒中检测到几种花青素衍生色素，其紫外-可见光谱与原始葡萄花青素不同。其中，大量检测到三种甲基花青素3-葡萄糖苷衍生色素，约占总花青素单糖苷含量的60%。这些色素通过液相色谱-质谱联用（LSI-MS）和核磁共振（NMR）（（1）H、双量子滤波相关谱（DQF-COSY）、旋转坐标系核Overhauser效应谱（ROESY）、异核单量子相干谱（HSQC）和异核多键相关谱（HMBC））技术进行分离、纯化和鉴定。主要色素是先前已鉴定的甲基花青素3-葡萄糖苷丙酮酸加合物，另外两种分别对应于其各自的乙酰基和香豆酰葡萄糖苷衍生物。后者首次在红葡萄酒中被报道。
