Zhang Na, Qi Yan, Zhang Hai-Jun, Wang Xiaoyun, Li Hongfei, Shi Yantong, Guo Yang-Dong
College of Horticulture, China Agricultural University Beijing, China.
College of Horticulture, China Agricultural UniversityBeijing, China; Beijing Agriculture Technology Extension StationBeijing, China.
Front Plant Sci. 2016 Dec 1;7:1804. doi: 10.3389/fpls.2016.01804. eCollection 2016.
Genistein (GNT), an isoflavone, is used in the clinical treatment of various health disorders. GNT is found in primary food source plants and some medical plants. However, studies on the functions of GNT in plants are rarely reported. In this study, we demonstrated that GNT plays an important role in promoting anthocyanin accumulation in red cabbage. GNT solutions (10, 20, 30, 40, and 50 mg/L) as foliar fertilizers were applied to red cabbage. Consequently, anthocyanin accumulation in red cabbage increased in a light-dependent manner. GNT solution at 30 mg/L exhibited the optimal effect on anthocyanin accumulation, which was twice that of the control. Quantitative real-time PCR analysis indicated that GNT application upregulated the expression of all structural genes, contributing to anthocyanin biosynthesis under light conditions. Under dark conditions, GNT exerted no significant promotive effect on anthocyanin accumulation; only early biosynthetic genes of anthocyanin biosynthesis responded to GNT. The promotive effect of GNT on anthocyanin biosynthesis is directly attributable to the regulation of structural gene expression. Transcription factors exhibited no response to GNT. The levels of anthocyanin in red cabbage positively correlated with the enzyme activities of antioxidant systems. This finding correlation suggested that the promotive effect of GNT on anthocyanin levels was correlated with improved antioxidant activity in the red cabbage.
染料木黄酮（GNT）是一种异黄酮，用于多种健康疾病的临床治疗。GNT存在于主要的食用植物和一些药用植物中。然而，关于GNT在植物中的功能研究鲜有报道。在本研究中，我们证明了GNT在促进红甘蓝花青素积累方面发挥着重要作用。将GNT溶液（10、20、30、40和50毫克/升）作为叶面肥施用于红甘蓝。结果，红甘蓝中的花青素积累呈光依赖性增加。30毫克/升的GNT溶液对花青素积累表现出最佳效果，是对照的两倍。实时定量PCR分析表明，施用GNT上调了所有结构基因的表达，有助于在光照条件下合成花青素。在黑暗条件下，GNT对花青素积累没有显著的促进作用；只有花青素生物合成的早期生物合成基因对GNT有反应。GNT对花青素生物合成的促进作用直接归因于对结构基因表达的调控。转录因子对GNT没有反应。红甘蓝中花青素的水平与抗氧化系统的酶活性呈正相关。这一相关性发现表明，GNT对花青素水平的促进作用与红甘蓝中抗氧化活性的提高有关。
