Menon T, Umamaheswari K, Kumarasamy N, Solomon S, Thyagarajan S P
Department of Microbiology, Dr A.L. Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India.
Acta Trop. 2001 Oct 22;80(2):151-4. doi: 10.1016/s0001-706x(01)00170-x.
A total of 46 strains of Candida were collected from HIV infected patients, of which 25 strains were isolated from patients with oral candidiasis, and 21 strains were from mouthwash samples of asymptomatic carriers. The most common species isolated was Candida albicans (73.9%), followed by Candida tropicalis (21.7%). In vitro susceptibility of the strains to fluconazole and itraconazole was tested using minimum inhibitory concentration (MIC) studies by agar dilution technique. Out of the 18 strains of C. albicans isolated from mouthwash samples, four were resistant to fluconazole whereas only two were resistant to itraconazole. Out of 16 strains of C. albicans isolated from oral lesions, one was resistant to fluconazole where as all were sensitive to itraconazole. Among the other species of Candida tested, C. tropicalis gave higher MIC values to both drugs than other species such as Candida guillermondii and Candida krusei. In vitro MIC values correlated well with in vivo responses in patients. Hence, itraconazole may be used as an alternative in the treatment of candidiasis, which does not respond to fluconazole therapy.
共从HIV感染患者中收集到46株念珠菌，其中25株分离自口腔念珠菌病患者，21株来自无症状携带者的漱口水样本。分离出的最常见菌种是白色念珠菌（73.9%），其次是热带念珠菌（21.7%）。采用琼脂稀释技术通过最低抑菌浓度（MIC）研究检测了这些菌株对氟康唑和伊曲康唑的体外敏感性。从漱口水样本中分离出的18株白色念珠菌中，4株对氟康唑耐药，而只有2株对伊曲康唑耐药。从口腔病变中分离出的16株白色念珠菌中，1株对氟康唑耐药，而所有菌株对伊曲康唑敏感。在检测的其他念珠菌属中，热带念珠菌对这两种药物的MIC值均高于其他菌种，如季也蒙念珠菌和克柔念珠菌。体外MIC值与患者的体内反应相关性良好。因此，对于氟康唑治疗无反应的念珠菌病，伊曲康唑可作为替代药物使用。
