[钙超载在大鼠急性水肿性胰腺炎向坏死性胰腺炎转变中的作用]

[Effects of calcium overload in the conversion of acute edematous pancreatitis to necrotizing pancreatitis in rats].

作者信息

Pu Q, Yan L, Shen J

机构信息

Department of General Surgery, First University Hospital, West China University of Medical Sciences, Chengdu 610041.

出版信息

Zhonghua Yi Xue Za Zhi. 1999 Feb;79(2):143-5.

PMID:11601024

Abstract

OBJECTIVE

To investigate the potential of pancreatic acinar cell calcium overload in the conversion of acute edematous pancreatitis (AEP) to necrotizing pancreatitis (ANP).

METHODS

Ninety-six Sprague-Dawley rats were randomized in three experimental groups. Sham-operated control (Group I) AEP (Group II) was induced by pancreatic duct ligation and intravenous injection of bombesin (100 micrograms/kg) and secretin (10 micrograms/kg). ANP (Group III) was induced same as group II but with a large dose of dextran 110,000(500 mg/kg) intravenously. Pancreatic acinar cell Ca2+ overload was studied using fluorescent probe Fura2. Cytosolic free Ca2+ concentration ([Ca2+]i) in isolated pancreatic acinar cells and Ca(2+)-ATPase activity in pancreatic cell plasma membranes were determined 1, 3, 6, 9 h respectively after treatment.

RESULTS

The results showed that pancreatic acinar cell [Ca2+]i was elevated at 1 h[(213 +/- 19) nmol/L, P < 0.05] and increased to (464 +/- 29) nmol/L at 6 h(P < 0.05) in rats with ANP, pancreatic cell plasma membrane Ca(2+)-ATPase activity decreased significantly from (29.8 +/- 0.4) nmol.min-1.mgp-1 at 1 h to (18.6 +/- 0.5) nmol.min-1.mgp-1 at 9 h in rats with ANP (P < 0.05).

CONCLUSIONS

In ischemia-induced conversion of AEP to ANP, there exists Ca2+ overload in the pancreatic acinar cells. The decreased pancreatic cell plasma membrane Ca(2+)-ATPase activity may be an important reason for acinar cell Ca2+ overload in the development of acute pancreatitis.

摘要

目的

探讨胰腺腺泡细胞钙超载在急性水肿性胰腺炎（AEP）向坏死性胰腺炎（ANP）转变中的作用。

方法

将96只Sprague-Dawley大鼠随机分为三个实验组。假手术对照组（I组）；AEP组（II组）通过结扎胰管并静脉注射蛙皮素（100微克/千克）和促胰液素（10微克/千克）诱导；ANP组（III组）诱导方法同II组，但静脉注射大剂量的右旋糖酐110,000（500毫克/千克）。使用荧光探针Fura2研究胰腺腺泡细胞Ca2+超载情况。分别在处理后1、3、6、9小时测定分离的胰腺腺泡细胞中的胞质游离Ca2+浓度（[Ca2+]i）以及胰腺细胞质膜中的Ca(2+)-ATP酶活性。

结果

结果显示，ANP大鼠胰腺腺泡细胞[Ca2+]i在1小时时升高[（213±19）纳摩尔/升，P<0.05]，并在6小时时升至（464±29）纳摩尔/升（P<0.05）；ANP大鼠胰腺细胞质膜Ca(2+)-ATP酶活性从1小时时的（29.8±0.4）纳摩尔·分钟-1·毫克蛋白-1显著降低至9小时时的（18.6±0.5）纳摩尔·分钟-1·毫克蛋白-1（P<0.05）。