清胰汤对急性胰腺炎大鼠细胞内Ca2+-Mg2+-ATP酶活性的影响。
Effect of Qingyitang on activity of intracellular Ca2+-Mg2+-ATPase in rats with acute pancreatitis.
作者信息
Qiu Ying, Li Yong-Yu, Li Shu-Guang, Song Bo-Gen, Zhao Gui-Fen
机构信息
Department of Pathophysiology, Medical School of Tongji University, Shanghai 200331, China.
出版信息
World J Gastroenterol. 2004 Jan;10(1):100-4. doi: 10.3748/wjg.v10.i1.100.
AIM
To study the change of intracellular calcium-magnesium ATPase (Ca(2+)-Mg(2+)-ATPase) activity in pancreas, liver and kidney tissues of rats with acute pancreatitis (AP), and to investigate the effects of Qingyitang (QYT) (Decoction for clearing the pancreas) and tetrandrine (Tet) and vitamin E (VitE) on the activity of Ca(2+)-Mg(2+)-ATPase.
METHODS
One hundred and five Sprague-Dawley rats were randomly divided into: normal control group, AP group, treatment group with QYT (1 ml/100 g) or Tet (0.4 ml/100 g) or VitE (100 mg/kg). AP model was prepared by a retrograde injection of sodium taurocholate into the pancreatic duct. Tissues of pancreas, liver and kidney of the animals were taken at 1 h, 5 h, 10 h respectively after AP induction, and the activity of Ca(2+)-Mg(2+)-ATPase was studied using enzyme-histochemistry staining. Meanwhile, the expression of Ca(2+)-Mg(2+)-ATPase of the tissues was studied by RT-PCR.
RESULTS
The results showed that the positive rate of Ca(2+)-Mg(2+)-ATPase in AP group (8.3%, 25%, 29.2%) was lower than that in normal control group (100%) in all tissues (P<0.01), the positive rate of Ca(2+)-Mg(2+)-ATPase in treatment group with QYT (58.3%, 83.3%, 83.3%), Tet (50.0%, 70.8%, 75.0%) and VitE (54.2%, 75.0%, 79.2%) was higher than that in AP group (8.3%, 25.0%, 29.2%) in all tissues (P<0.01). RT-PCR results demonstrated that in treatment groups Ca(2+)-Mg(2+)-ATPase gene expression in pancreas tissue was higher than that in AP group at the observing time points, and the expression at 5 h was higher than that at 1 h. The expression of Ca(2+)-Mg(2+)-ATPase in liver tissue was positive, but without significant difference between different groups.
CONCLUSION
The activity and expression of intracellular Ca(2+)-Mg(2+)-ATPase decreased in rats with AP, suggesting that Ca(2+)-Mg(2+)-ATPase may contribute to the occurrence and development of cellular calcium overload in AP. QYT, Tet and VitE can increase the activity and expression of Ca(2+)-Mg(2+)-ATPase and may relieve intracellular calcium overload to protect the tissue and cells from injuries.
目的
研究急性胰腺炎（AP）大鼠胰腺、肝脏和肾脏组织中细胞内钙镁ATP酶（Ca(2+)-Mg(2+)-ATPase）活性的变化，并探讨清胰汤（QYT）、粉防己碱（Tet）和维生素E（VitE）对Ca(2+)-Mg(2+)-ATPase活性的影响。
方法
将105只Sprague-Dawley大鼠随机分为：正常对照组、AP组、清胰汤治疗组（1 ml/100 g）、粉防己碱治疗组（0.4 ml/100 g）和维生素E治疗组（100 mg/kg）。通过逆行胰胆管注射牛磺胆酸钠制备AP模型。在诱导AP后1 h、5 h、10 h分别取动物的胰腺、肝脏和肾脏组织，采用酶组织化学染色法研究Ca(2+)-Mg(2+)-ATPase的活性。同时，通过RT-PCR研究组织中Ca(2+)-Mg(2+)-ATPase的表达。
结果
结果显示，AP组所有组织中Ca(2+)-Mg(2+)-ATPase的阳性率（8.3%、25%、29.2%）低于正常对照组（100%）（P<0.01），清胰汤治疗组（58.3%、83.3%、83.3%）、粉防己碱治疗组（50.0%、70.8%、75.0%）和维生素E治疗组（54.2%、75.0%、79.2%）所有组织中Ca(2+)-Mg(2+)-ATPase的阳性率高于AP组（8.3%、25.0%、29.2%）（P<0.01）。RT-PCR结果表明，在观察时间点，治疗组胰腺组织中Ca(2+)-Mg(2+)-ATPase基因表达高于AP组，且5 h时的表达高于1 h时。肝脏组织中Ca(2+)-Mg(2+)-ATPase的表达为阳性，但不同组间无显著差异。
结论
AP大鼠细胞内Ca(2+)-Mg(2+)-ATPase的活性和表达降低，提示Ca(2+)-Mg(2+)-ATPase可能参与AP细胞钙超载的发生和发展。清胰汤、粉防己碱和维生素E可提高Ca(2+)-Mg(2+)-ATPase的活性和表达，可能减轻细胞内钙超载，保护组织和细胞免受损伤。
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