[线粒体跨膜电位降低是血液透析患者外周血单核细胞凋亡的早期事件]

[Reduction of mitochondrial transmembrane potential constitutes an early event of peripheral blood monocytes apoptosis in hemodialysis patients].

作者信息

Jin H M, Xu Q J, Wu Z J

机构信息

Department of Nephrology, Shanghai Medical University, Shanghai First People's Hospital, Shanghai 200080.

出版信息

Zhonghua Yi Xue Za Zhi. 1999 Mar;79(3):190-3.

PMID:11601038

Abstract

OBJECTIVE

To investigate the relationship between monocyte apoptosis and mitochondria function in hemodialysis (HD) patients.

METHODS

Fluorescein labeled Annexin-V was used to detect the phosphatidyserine (PS) of apoptosis cells, and mitochondrial transmembrane potential was studied by 3,3'-dihexyloxacarbocyanine iodide, Di0C6(3), and analyzed by flow cytometry. Swelling of mitochondrial permeability transition (PT) was recorded by spectrophotometer.

RESULTS

Spontaneous apoptosis of cultured monocytes increased at 24, 72 hours in HD patients (41.4 +/- 12.2)%, (22.0 +/- 3.0)% as compared with controls (20.6 +/- 8.8)%, (9.8 +/- 2.1)%. Freshly isolated monocytes in HD patients had little apoptosis, but mitochondrial transmembrane potential was reduced to half of disruption (43 +/- 9)%. As to swelling of PT, those freshly isolated monocytes mitochondria in HD patients experienced overwhelming opening of PT, (80 +/- 5.6)%.

CONCLUSION

Swelling of PT and breakdown of mitochondrial transmembrane potential constitute the early events of monocytes apoptosis in HD patients, which could contribute to the impaired monocytes function.

摘要

目的

探讨血液透析（HD）患者单核细胞凋亡与线粒体功能之间的关系。

方法

采用荧光素标记的膜联蛋白-V检测凋亡细胞的磷脂酰丝氨酸（PS），用3,3'-二己基氧羰花青碘化物（Di0C6(3)）研究线粒体跨膜电位，并通过流式细胞术进行分析。用分光光度计记录线粒体通透性转换（PT）肿胀情况。

结果

HD患者培养的单核细胞在24小时、72小时时自发凋亡增加，分别为（41.4±12.2）%、（22.0±3.0）%，而对照组分别为（20.6±8.8）%、（9.8±2.1）%。HD患者新鲜分离的单核细胞凋亡较少，但线粒体跨膜电位降低至破坏程度的一半（43±9）%。至于PT肿胀，HD患者新鲜分离的单核细胞线粒体PT大量开放，为（80±5.6）%。