[自发性高血压大鼠的肌浆网钙离子处理蛋白与左心室肥厚]

[The sarcoplasmic reticulum Ca2+ handling protein and left ventricular hypertrophy in SHR].

作者信息

Zhu B, Zhang J, Liu N

机构信息

Department of Cardiology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029.

出版信息

Zhonghua Yi Xue Za Zhi. 1999 Apr;79(4):286-8.

PMID:11601055

Abstract

OBJECTIVE

To elucidate the role of myocardial angiotensin II and sarcoplasmic reticulum (SR) Ca2+ handling protein (ryanodine receptor and Ca2+ ATPase) in the process of left ventricular hypertrophy.

METHODS

The myocardial angiotensin II level, Bmax and Kd value of ryanodine receptor and calcium ATPase activity in SR were determined among 3 groups: spontaneously hypertensive rats (SHR), SHR treated with perindopril and WKY.

RESULTS

The blood pressure (BP) of the SHR group, the ratio of left ventricular weight to body weight (LVW/BW) and the myocardial angiotensin II level were higher than those of the WKY group (P < 0.01), Perindopril decreased the BP(P < 0.01) and the myocardial angiotensin II level, and reversed the LVW/BW of the SHR group (P < 0.05). Compared to the WKY group, the Bmax value of cardiac ryanodine receptor in the SHR group was increased (232 +/- 27) fmol.mg-1 vs (173 +/- 18) fmol.mg-1, (P < 0.01), but there was no difference in Kd value (P > 0.05). The SR calcium ATPase activity was also decreased in the SHR group [(0.52 +/- 0.11) mumol.g-1.min-1 vs (1.03 +/- 0.22) mumol.g-1.min-1, P < 0.01]. In the SHR perindopril treatment group, the Bmax of ryanodine receptor was decreased [(191 +/- 23) fmol.mg-1, P < 0.05]. There was no difference in Kd value. The calcium ATPase activity was increased [(0.82 +/- 0.28) mumol.g-1.min-1, P < 0.05)].

CONCLUSION

The left ventricular hypertrophy is closely related to the myocardial angiotensin II and the SR calcium handling protein.

摘要

目的

阐明心肌血管紧张素II及肌浆网（SR）钙处理蛋白（兰尼碱受体和钙ATP酶）在左心室肥厚过程中的作用。

方法

测定3组动物的心肌血管紧张素II水平、兰尼碱受体的Bmax和Kd值以及SR中的钙ATP酶活性，这3组分别为自发性高血压大鼠（SHR）、用培哚普利治疗的SHR和WKY大鼠。

结果

SHR组的血压（BP）、左心室重量与体重之比（LVW/BW）及心肌血管紧张素II水平均高于WKY组（P<0.01）。培哚普利降低了血压（P<0.01）和心肌血管紧张素II水平，并使SHR组的LVW/BW恢复正常（P<0.05）。与WKY组相比，SHR组心脏兰尼碱受体的Bmax值升高[（232±27）fmol.mg-1对（173±18）fmol.mg-1，P<0.01]，但Kd值无差异（P>0.05）。SHR组的SR钙ATP酶活性也降低[（0.52±0.11）μmol.g-1.min-1对（1.03±0.22）μmol.g-1.min-1，P<0.01]。在SHR培哚普利治疗组，兰尼碱受体的Bmax降低[（191±23）fmol.mg-1，P<0.05]。Kd值无差异。钙ATP酶活性升高[（0.82±0.28）μmol.g-1.min-1，P<0.05]。