Hu J, Lu L, Chen Y
Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001.
Zhonghua Xue Ye Xue Za Zhi. 1999 Feb;20(2):73-5.
In order to clarify the clinical and laboratory features of adult T cell leukemia (ATL): morphology, immunology, cytogenetics, serology and molecular biology.
Indirect immunofluorescence assay and ELISA were used to detect serum HTLV-I antibody. The HTLV-I provirus sequence were amplified by PCR and confirmed by liquid hybridization.
Nine cases of ATL were diagnosed. The major clinical manifestation was lymph node enlargement found in all patients. Skin involvement and osteolysis were not frequent. The characteristic finding was leukemic cells with highly indented or lobulated flower-like nuclei in peripheral blood and bone marrow. ATL cells were CD2, CD3, CD4, CD25 positive and CD8 negative. No specific chromosome abnormality or HLA type was found. Seven of 8 patients examined had HTLV-I antibody. The HTLV-I provirus genome sequence integrated into host cell DNA was amplified by PCR and confirmed by liquid hybridization. All of these results showed that HTLV-I was also the etiological agent of ATL in China. One of the 9 cases of ATL was classified as lymphoma type, one as chronic-type, and the rest as acute type.
为阐明成人T细胞白血病（ATL）的临床和实验室特征，包括形态学、免疫学、细胞遗传学、血清学及分子生物学特征。
采用间接免疫荧光法和酶联免疫吸附测定法检测血清HTLV-I抗体。通过聚合酶链反应（PCR）扩增HTLV-I前病毒序列，并经液相杂交确认。
确诊9例ATL。主要临床表现为所有患者均出现淋巴结肿大。皮肤受累和骨质溶解不常见。特征性表现为外周血和骨髓中白血病细胞具有高度凹陷或分叶的花状核。ATL细胞CD2、CD3、CD4、CD25呈阳性，CD8呈阴性。未发现特异性染色体异常或HLA类型。8例接受检测的患者中有7例HTLV-I抗体呈阳性。通过PCR扩增并经液相杂交确认了整合到宿主细胞DNA中的HTLV-I前病毒基因组序列。所有这些结果表明，HTLV-I也是中国ATL的病原体。9例ATL中，1例为淋巴瘤型，1例为慢性型，其余为急性型。
