Li Z, Wen Z, Han J
Department of Physiology, Hunan Medical University, ChangSha 410078.
Zhonghua Xue Ye Xue Za Zhi. 1999 Mar;20(3):130-3.
To study the stimulating effect of thrombin on the expression of tissue factor (TF) activity in cultured bovine aortic endothelial cells (BAEC) and to elucidate if it is associated with the PKC system.
The BAECs were cultured after the Schwartz's method and one stage clotting time assay was used to measure TF activity in BAEC lysates. The TF activity was identified by EDGR-VIIa and a monoclonal antibody against TF.
Thrombin enhanced the expression of TF activity in cultured BAEC and the effect was dose- (r = 0.78, P < 0.05, n = 8) and time-dependent (r = 0.88, P < 0.05, n = 8). PMA, a PKC activator, also induced the expression of TF activity in BAEC. H7, a protein kinase C inhibitor, inhibited the inductive effects of PMA and thrombin on TF in BAEC.
Thrombin can induce the expression of TF activity in cultured BAEC and the effect depends on PKC pathway.
研究凝血酶对培养的牛主动脉内皮细胞（BAEC）中组织因子（TF）活性表达的刺激作用，并阐明其是否与蛋白激酶C（PKC）系统相关。
采用施瓦茨方法培养BAEC，并用一期凝血时间测定法测量BAEC裂解物中的TF活性。TF活性通过EDGR-VIIa和抗TF单克隆抗体进行鉴定。
凝血酶增强了培养的BAEC中TF活性的表达，且该作用呈剂量依赖性（r = 0.78，P < 0.05，n = 8）和时间依赖性（r = 0.88，P < 0.05，n = 8）。PKC激活剂佛波酯（PMA）也诱导BAEC中TF活性的表达。蛋白激酶C抑制剂H7抑制了PMA和凝血酶对BAEC中TF的诱导作用。
凝血酶可诱导培养的BAEC中TF活性的表达，且该作用依赖于PKC途径。
