[凝血酶对培养的牛主动脉内皮细胞组织因子活性的影响及其与蛋白激酶C途径的相关性]

[The effect of thrombin on tissue factor activity in cultured bovine aortic endothelial cells and its correlation with PKC pathway].

作者信息

Li Z, Wen Z, Han J

机构信息

Department of Physiology, Hunan Medical University, ChangSha 410078.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 1999 Mar;20(3):130-3.

PMID:11601238

Abstract

OBJECTIVE

To study the stimulating effect of thrombin on the expression of tissue factor (TF) activity in cultured bovine aortic endothelial cells (BAEC) and to elucidate if it is associated with the PKC system.

METHODS

The BAECs were cultured after the Schwartz's method and one stage clotting time assay was used to measure TF activity in BAEC lysates. The TF activity was identified by EDGR-VIIa and a monoclonal antibody against TF.

RESULTS

Thrombin enhanced the expression of TF activity in cultured BAEC and the effect was dose- (r = 0.78, P < 0.05, n = 8) and time-dependent (r = 0.88, P < 0.05, n = 8). PMA, a PKC activator, also induced the expression of TF activity in BAEC. H7, a protein kinase C inhibitor, inhibited the inductive effects of PMA and thrombin on TF in BAEC.

CONCLUSION

Thrombin can induce the expression of TF activity in cultured BAEC and the effect depends on PKC pathway.

摘要

目的

研究凝血酶对培养的牛主动脉内皮细胞（BAEC）中组织因子（TF）活性表达的刺激作用，并阐明其是否与蛋白激酶C（PKC）系统相关。

方法

采用施瓦茨方法培养BAEC，并用一期凝血时间测定法测量BAEC裂解物中的TF活性。TF活性通过EDGR-VIIa和抗TF单克隆抗体进行鉴定。

结果

凝血酶增强了培养的BAEC中TF活性的表达，且该作用呈剂量依赖性（r = 0.78，P < 0.05，n = 8）和时间依赖性（r = 0.88，P < 0.05，n = 8）。PKC激活剂佛波酯（PMA）也诱导BAEC中TF活性的表达。蛋白激酶C抑制剂H7抑制了PMA和凝血酶对BAEC中TF的诱导作用。