Han J Z, Li J C, Qu S L, He S L
Department of Physiology, Hunan Medical University, Changsha.
Sheng Li Xue Bao. 1997 Dec;49(6):675-8.
The purpose of the present study was to elucidate the roles that protein kinase C (PKC) and calcium played in the tissue factor (TF) synthesis and tissue factor pathway inhibitory (TFPI) release in human umbilic vein endothelial cells (HUVEC). A23187 was used to represent calcium ionophore and phorbol 12-myristate 13-acetate (PMA) as that of PKC activator. TF activity in the lysed HUVEC was measured using one stage clotting assay. TFPI activity in the conditioned medium of HUVEC was assessed by the two-step chromogenic method. The results showed that the TF activities in A23187, PMA and A23187 + PMA groups were remarkably higher (P < 0.01) than that in control. Among the three treated groups, the TF activities in both A23187 group and A23187 + PMA group were lower than that in the PMA group (P < 0.05), but the difference between the former two groups was statically insignificant (P > 0.05). In contrast to the control group, the TFPI activity in the A23187 group was not statistically different (P > 0.05). However, the TFPI activities in the PMA group and the A23187 + PMA group were markedly higher than those in the control group and the A23187 group (P < 0.01). These findings indicate that PKC and calcium ion promote TF synthesis in HUVEC but the effect of the former is stronger than that of the latter, and that the release of TFPI from HUVEC is facilitated by PKC and not significantly affected by calcium ion.
本研究的目的是阐明蛋白激酶C（PKC）和钙在人脐静脉内皮细胞（HUVEC）中组织因子（TF）合成及组织因子途径抑制物（TFPI）释放过程中所起的作用。用A23187代表钙离子载体，佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）代表PKC激活剂。采用一步凝固法测定裂解的HUVEC中的TF活性。通过两步显色法评估HUVEC条件培养基中的TFPI活性。结果显示，A23187组、PMA组和A23187 + PMA组的TF活性显著高于对照组（P < 0.01）。在这三个处理组中，A23187组和A23187 + PMA组的TF活性均低于PMA组（P < 0.05），但前两组之间的差异无统计学意义（P > 0.05）。与对照组相比，A23187组的TFPI活性无统计学差异（P > 0.05）。然而，PMA组和A23187 + PMA组的TFPI活性显著高于对照组和A23187组（P < 0.01）。这些发现表明，PKC和钙离子可促进HUVEC中TF的合成，但前者的作用强于后者，并且PKC可促进HUVEC释放TFPI，而钙离子对其影响不显著。
