Liu L, Teng G, Zhang D, Song J, He S, Guo J, Fang W
Institute of Medical Science, Nanjing Railway Medical College, Nanjing 210009, China.
Chin Med J (Engl). 1999 Jul;112(7):632-6.
To evaluate the toxic response to intrahepatic arterial administration of radioactive phosphorus-32 glass microspheres (32P-GMS) in domestic pigs.
Through selective catheterization of hepatic artery, 32P-GMS was infused to 5 healthy domestic pigs in a dosage equivalent to the therapeutic dose for human being, and 31P-GMS was infused to other 5 healthy domestic pigs. Two pigs served as the whole course blank controls. One pig from each group was surrendered to euthanasia at week 1, 2, 4, 8 and 16, respectively, and liver biopsies were performed on the rest of pigs at the corresponding time points. Liver tissues from different sites were taken for light and electron microscopy. The ultrastructural histopathological changes were evaluated semiquantitatively.
The accumulative amount of 32P-GMS in the target tissue attained more than 90% of the total dose administrated. Histologically, abnormal hepatocytes were easily found at week 1 or 2. At week 4 they were less than at week 1 or 2, and endothelium of the sinuses were damaged prominently. At week 8 they were scarcely seen, and liver tissue recovered gradually. The histological features of liver tissue restored to normal at week 16. Semiquantitative analysis of ultrastructural morphology in the experimental group showed no statistical difference (P > 0.50) between the nuclear abnormality (Nabn) and mitochrondrial variability (Mvar) at week 1 or 2, but revealed prominent difference (P < 0.01, P < 0.001) as compared with those in other groups. In the experimental group the Nabn in tissues showed no significant difference (P > 0.20) between week 8 and week 16.
32P-GMS internal irradiation at the dosage equivalent to human therapeutic dosage exerts reversible injury to domestic pig liver tissue, and it takes more than 8 weeks for the injured liver tissue to recover.
评估家猪肝动脉内注射放射性磷-32玻璃微球（32P-GMS）的毒性反应。
通过肝动脉选择性插管，将相当于人类治疗剂量的32P-GMS注入5只健康家猪体内，另将31P-GMS注入另外5只健康家猪体内。2只家猪作为全程空白对照。每组分别在第1、2、4、8和16周处死1只家猪，其余家猪在相应时间点进行肝脏活检。取不同部位的肝组织进行光镜和电镜检查。对超微结构组织病理学变化进行半定量评估。
32P-GMS在靶组织中的累积量达到给药总量的90%以上。组织学上，在第1周或第2周容易发现异常肝细胞。第4周时比第1周或第2周减少，肝血窦内皮显著受损。第8周时几乎未见，肝组织逐渐恢复。第16周时肝组织的组织学特征恢复正常。实验组超微结构形态的半定量分析显示，第1周或第2周时核异常（Nabn）和线粒体变异（Mvar）之间无统计学差异（P>0.50），但与其他组相比有显著差异（P<0.01，P<0.001）。实验组组织中的Nabn在第8周和第16周之间无显著差异（P>0.20）。
相当于人类治疗剂量的32P-GMS内照射对家猪肝组织造成可逆性损伤，受损肝组织恢复需要8周以上时间。
