东方田鼠对日本血吸虫感染天然抵抗力的免疫学特性

Immunological characteristics of natural resistance in Microtus fortis to infection with Schistosoma japonicum.

作者信息

He Y, Luo X, Zhang X, Yu X, Lin J, Li Y, Li Y, Liu S

机构信息

Hunan Institute of Parasitic Diseases, Hunan 414000, China.

出版信息

Chin Med J (Engl). 1999 Jul;112(7):649-54.

PMID:11601263

Abstract

OBJECTIVE

To explore the immunological characteristics of natural resistance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area.

METHODS

Passive transfer of sera from uninfected laboratory bred MF (BMF) to albinao mice (AM) was performed to observe the acquired protection. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoblotting (ELIB) methods were used to recognize 4 different life-cycle stage antigens of S. japonicum by sera from wild MF (WMF), BMF, BMF13 and BMF19. Tests were also performed on in vitro killing effect of sera and/or lymphocytes from BMF and WMF to schistosomulae; quantitative determination of C3 and C4 by immunoturbidometry, and interleukin-4 (IL-4) and antibodies to the 4 stage antigens in sera from WMF, BMF and infected BMF by ELISA.

RESULTS

Compared with the control group, stool eggs per gram (EPG) of AM in the test group was significantly reduced by 81.54%, miracidium hatching rate, by 50.67%, liver egg counts, by 72.07%, the diameter of hepatic egg granuloma, by 70.39 microns. Western blotting probed with the 4 MF sera all revealed 7 specific bands for SSA, 3 for AWA and SEA, but none for CA antigens. The sera and/or lymphocytes from WMF and BMF gave obvious killing effects on schistosomulae with an adjusted death rate of 64.12%-78.83%. The levels of "natural antibodies" produced by MF to S. japonicum were in the following order: anti-SSA > anti-AWA > anti-SEA > anti-CA, all of which increased significantly after the infection. Serum levels of C3, C4 and IL-4 in uninfected BMF were significantly higher than those in AM. After infection, levels of C3 and C4 were further increased respectively by 72.83% and 295.49% in the 4th week and IL-4 by 303.83% in the 9th day.

CONCLUSIONS

Immunological characteristics of innate resistance in M. fortis to S. japonicum infection were existed with no significant difference between WMF and BMF.

摘要

目的

探讨洞庭湖区东方田鼠对日本血吸虫感染天然抵抗力的免疫学特性。

方法

将未感染的实验室饲养东方田鼠（BMF）的血清被动转移至白化小鼠（AM），观察获得性保护作用。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和酶联免疫印迹法（ELIB），用野生东方田鼠（WMF）、BMF、BMF13和BMF19的血清识别日本血吸虫4个不同生活史阶段的抗原。还检测了BMF和WMF的血清和/或淋巴细胞对血吸虫毛蚴的体外杀伤作用；用免疫比浊法定量测定C3和C4，并用ELISA法检测WMF、BMF和感染的BMF血清中白细胞介素-4（IL-4）和4种阶段抗原的抗体。

结果

与对照组相比，试验组AM每克粪便虫卵数（EPG）显著降低81.54%，毛蚴孵化率降低50.67%，肝脏虫卵数降低72.07%，肝虫卵肉芽肿直径减小70.39微米。用4种东方田鼠血清进行蛋白质印迹分析，均显示出7条针对可溶性虫卵抗原（SSA）的特异性条带，3条针对成虫水溶性抗原（AWA）和虫卵抗原（SEA）的特异性条带，但未显示针对尾蚴抗原（CA）的条带。WMF和BMF的血清和/或淋巴细胞对血吸虫毛蚴有明显杀伤作用，校正死亡率为64.12%-78.83%。东方田鼠产生的针对日本血吸虫的“天然抗体”水平依次为：抗SSA＞抗AWA＞抗SEA＞抗CA，感染后均显著升高。未感染的BMF血清中C3、C4和IL-4水平显著高于AM。感染后第4周C3和C4水平分别进一步升高72.83%和295.49%，第9天IL-4升高303.83%。