Gong S, Zeng X, Yan Z, Wang J
Department of Otorhinolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022, China.
Chin Med J (Engl). 1999 Aug;112(8):728-30.
To investigate the role of peripheral immunocompetent lymphocytes during the immune response in the inner ear.
Sensitized lymphocytes from peripheral blood, neck lymph nodes and spleen of three groups of donor animals, i.e., group A immunized intradermally with keyhole limpet hemocyanine (KLH) and both inner ears challenged with KLH, group B received only KLH intradermally without inner ear challenge, and group C used as control, were isolated and labelled with 51Cr. The labelled lymphocytes were injected intravenously into the recipients. They were immunized intradermally with right inner ears challenged with KLH. Twenty to 24 h later, the radioactivities in the temporal bones of the recipients were assayed to detect the infiltration of labelled cells.
In groups A and B, the radioactivity in the right temporal bones was higher than that in the left temporal bones (P < 0.05). The highest labelling radioactivity was detected in the right temporal bones of group A. The lowest migration of 51Cr-labelled cells to the inner ear was found in group C. The ratio of radioactivity between right (challenged) and left (unchallenged) inner ears was higher in group A than in groups B and C, with the difference being significant (P < 0.05).
The immune reaction in response to antigenic stimulation in the inner ear is immunologically modulated by the peripheral systemic circulation.
探讨外周免疫活性淋巴细胞在内耳免疫反应中的作用。
从三组供体动物的外周血、颈部淋巴结和脾脏中分离出致敏淋巴细胞，即A组动物皮内注射血蓝蛋白（KLH）并双耳均用KLH攻击，B组仅皮内注射KLH而不进行内耳攻击，C组作为对照，然后用51Cr标记。将标记的淋巴细胞静脉注射到受体动物体内。受体动物皮内免疫，右耳用KLH攻击。20至24小时后，检测受体动物颞骨中的放射性，以检测标记细胞的浸润情况。
A组和B组中，右颞骨的放射性高于左颞骨（P<0.05）。A组右颞骨检测到的标记放射性最高。C组中51Cr标记细胞向内耳的迁移最少。A组右耳（受攻击）与左耳（未受攻击）内耳的放射性比值高于B组和C组，差异有统计学意义（P<0.05）。
内耳对抗原刺激的免疫反应受到外周体循环的免疫调节。
