Gloddek B, Ryan A F, Harris J P
Department of ENT, Medizinische Hochschule, Hannover, Germany.
Acta Otolaryngol. 1991;111(6):1051-9. doi: 10.3109/00016489109100755.
The migration of lymphocytes to the inner ear was studied during an immune response in the cochlea. Sensitized lymphocytes from peripheral blood, neck lymph nodes and spleen from strain 13 inbred guinea pigs were labelled with 51Cr and injected intravenously into strain 13 recipients undergoing an inner ear immune response. Eighteen hours later the temporal bones and immune organs of the recipients were assayed for radioactivity to detect the infiltration of labelled cells. In addition autoradiography was performed to localize labelled cells in the inner ear. More lymphocytes from the peripheral blood entered the inner ear during the immune response than spleen or lymph node cells. This indicates that the inner ear comes under the immuno-surveillance of the peripheral circulation in response to antigenic stimulation. Most labelled lymphocytes were observed in the basal turn of the scala tympani and in and around the spiral modiolar vein of the challenged cochlea. A few cells were seen also in the control cochleas but almost all where inside the blood vessels. This pattern suggests that the blood vessels of the spiral modiolar vein are the initial site through which lymphocytes entered the inner ear.
在耳蜗的免疫反应过程中，研究了淋巴细胞向内耳的迁移情况。用51Cr标记来自13系近交豚鼠外周血、颈部淋巴结和脾脏的致敏淋巴细胞，并将其静脉注射到正在经历内耳免疫反应的13系受体动物体内。18小时后，检测受体动物的颞骨和免疫器官的放射性，以检测标记细胞的浸润情况。此外，还进行了放射自显影，以在内耳中定位标记细胞。在免疫反应期间，来自外周血的淋巴细胞进入内耳的数量比来自脾脏或淋巴结的细胞更多。这表明，在内耳受到抗原刺激时，内耳处于外周循环的免疫监视之下。在受攻击耳蜗的鼓阶基底转以及螺旋蜗轴静脉内和周围观察到了大多数标记淋巴细胞。在对照耳蜗中也可见少数细胞，但几乎所有细胞都在血管内。这种模式表明，螺旋蜗轴静脉的血管是淋巴细胞进入内耳的初始部位。
