紫外线照射的尿刊酸对T淋巴细胞分泌白细胞介素-10的影响。

Effects of ultraviolet-irradiated urocanic acid on IL-10 secreted by T lymphocytes.

作者信息

Bi Z, Xia J, Ni L

机构信息

Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, No. 300 Guang Zhou Road, Nanjing 210029, China.

出版信息

Chin Med J (Engl). 1999 Jun;112(6):509-11.

PMID:11601328

Abstract

OBJECTIVE

To further investigate the effects of ultraviolet (UV)-irradiated urocanic acid (UCA) on T lymphocytes, the effects of cis-UCA on T lymphocyte IL-10 secretion was studied.

METHODS

Four groups of normal lymphocytes were cultured in RPMI-1640 containing 15% fetal calf serum. Besides negative and phytohemagglutinin (PHA) positive control, the cis-UCA and trans-UCA groups were set. The number of lymphocytes was 1.5 x 10(6). PHA was 0.05 mg/ml in each group. Both cis-UCA and trans-UCA were detected by 1.00, 0.75, 0.50 and 0.20 mmol/L respective concentrations. IL-10 was detected by ELISA method after 48 hours' culture. IL-10 mRNA expression level was assayed by RT-PCR.

RESULTS

The results indicated cis-UCA could reduce IL-10 secreted by T lymphocytes much more than trans-UCA (t = 2.91, P < 0.05) could. It was also found that the concentrations of cis-UCA were negatively correlated with IL-10 levels (r = -0.99, tr = 9.23, P < 0.01). The IL-10 mRNA expression of T lymphoctytes was inhibited by cis-UCA.

CONCLUSION

Our investigations revealed that cis-UCA, a major UV receptor in the stratum comeum of epidermis, could inhibit T lymphoctes secreting IL-10 by suppressing IL-10 mRNA expression, finally affects the humoral immune response.

摘要

目的

为进一步研究紫外线（UV）照射后的尿刊酸（UCA）对T淋巴细胞的影响，本研究探讨了顺式尿刊酸（cis-UCA）对T淋巴细胞白细胞介素-10（IL-10）分泌的影响。

方法

将四组正常淋巴细胞培养于含15%胎牛血清的RPMI-1640培养基中。除阴性对照组和植物血凝素（PHA）阳性对照组外，另设顺式尿刊酸组和反式尿刊酸组。淋巴细胞数量为1.5×10⁶个。每组PHA浓度为0.05mg/ml。顺式尿刊酸和反式尿刊酸分别采用1.00、0.75、0.50和0.20mmol/L的浓度。培养48小时后，采用酶联免疫吸附测定（ELISA）法检测IL-10。采用逆转录-聚合酶链反应（RT-PCR）检测IL-10 mRNA表达水平。