顺式尿刊酸单克隆抗体的特性：通过免疫测定法检测受辐照小鼠血清中的顺式尿刊酸。
Characterization of a monoclonal antibody to cis-urocanic acid: detection of cis-urocanic acid in the serum of irradiated mice by immunoassay.
作者信息
Moodycliffe A M, Norval M, Kimber I, Simpson T J
机构信息
Department of Medical Microbiology, University of Edinburgh Medical School, U.K.
出版信息
Immunology. 1993 Aug;79(4):667-72.
Cis-urocanic acid (cis-UCA), which is formed from the naturally occurring trans-isomer on ultraviolet (UV) irradiation, has been suggested as a photoreceptor for and mediator of the suppressive effects of UV irradiation on systemic immune responses. Trans-UCA is located predominantly in the stratum corneum, and the extent of isomerization to cis-UCA may be analysed by high-performance liquid chromatography (HPLC) of skin extracts. Such an analysis is not suitable for other tissues. In this study a murine monoclonal antibody to cis-UCA was prepared and tested by ELISA using UCA isomers conjugated to protein as antigens. The interaction of the antibody with structural analogues of UCA was assessed by competitive inhibition ELISA which indicated that the antibody had a high specificity for cis-UCA. Screening of sera at various times after UVB irradiation of mice by competitive inhibition ELISA using the monoclonal antibody showed that cis-UCA was present, probably in an unbound form, for at least 2 days after the exposure. Thus, cis-UCA produced in the epidermis following UVB irradiation reaches the serum a few hours later. The implications of this finding for the generation of suppressed immune responses are discussed.
顺式尿刊酸（cis-UCA）由天然存在的反式异构体经紫外线（UV）照射形成，被认为是UV照射对全身免疫反应抑制作用的光感受器和介质。反式-UCA主要位于角质层，可通过对皮肤提取物进行高效液相色谱（HPLC）分析来测定其向顺式-UCA的异构化程度。这种分析方法不适用于其他组织。在本研究中，制备了一种针对顺式-UCA的鼠单克隆抗体，并以与蛋白质偶联的UCA异构体作为抗原，通过酶联免疫吸附测定（ELISA）进行检测。通过竞争性抑制ELISA评估该抗体与UCA结构类似物的相互作用，结果表明该抗体对顺式-UCA具有高度特异性。使用该单克隆抗体通过竞争性抑制ELISA对紫外线B（UVB）照射小鼠后不同时间的血清进行筛查，结果显示顺式-UCA在暴露后至少2天内存在，可能以未结合形式存在。因此，UVB照射后表皮中产生的顺式-UCA在数小时后到达血清。本文讨论了这一发现对免疫反应抑制产生的影响。
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