Tepedino V, Berrini A, Borromeo V, Gaggioli D, Cantoni C, Manzoni P, Secchi C
University of Milan, Istituto di Ispezione degli Alimenti di Origine Animale, Italy.
J AOAC Int. 2001 Sep-Oct;84(5):1600-7.
Isoelectric focusing in polyacrylamide gel was used to establish an identification archive of fish species belonging to the orders Pleuronectiformes, or flat fish, and Gadiformes, or gadoid fish. The 2 orders include species of different commercial value and interest that are frequently requested in European fish markets, but are susceptible to substitution either because they are morphologically similar or because they arrive on the markets already filleted or sliced. The sarcoplasmic protein profiles are species-specific and reproducible. The use of densitometry and image analysis coupled with a simple computer program overcomes the subjective evaluation of the patterns, making it possible to identify species correctly.
采用聚丙烯酰胺凝胶等电聚焦法建立了鲽形目（即比目鱼）和鳕形目（即鳕鱼）鱼类的鉴定档案。这两个目包含了具有不同商业价值且备受关注的物种，在欧洲鱼类市场上经常被需求，但由于形态相似，或者上市时已是鱼片或鱼块，容易被替代。肌浆蛋白谱具有物种特异性且可重复。使用光密度测定法和图像分析，再结合一个简单的计算机程序，克服了对图谱的主观评估，从而能够正确鉴定物种。
